Molybdate inhibition of sulphate reduction in two-phase anaerobic digestion

Several researchers have used molybdate (MoO4 2-) for the selective inhibition of sulphate reducing bacteria (SRB) in anaerobic digestion. The feasibility of using MoO4 2- for the control of sulphate reduction in biological reactors, however, is not well established as some published results have al...

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Main Authors: Isa, M.H., Anderson, G.K.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.1280 /
Published: 2005
Subjects:
TD Environmental technology. Sanitary engineering
Online Access: http://eprints.utp.edu.my/1280/1/Molybdate_inhibition_of_sulphate_reduction_in_two-phase_anaerobic_digestion.pdf
http://eprints.utp.edu.my/1280/
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spelling utp-eprints.12802017-03-20T02:32:26Z Molybdate inhibition of sulphate reduction in two-phase anaerobic digestion Isa, M.H. Anderson, G.K. TD Environmental technology. Sanitary engineering Several researchers have used molybdate (MoO4 2-) for the selective inhibition of sulphate reducing bacteria (SRB) in anaerobic digestion. The feasibility of using MoO4 2- for the control of sulphate reduction in biological reactors, however, is not well established as some published results have also indicated an adverse effect on methane producing bacteria (MPB). Two possible reasons may be attributed for this observation, namely: (a) chronic inhibition of MPBs by MoO4 2- and (b) preferential acclimatisation of acidogenic bacteria to MoO4 2-. A two-phase anaerobic digestion experiment was conducted to establish the reason for the cessation of methanogenesis as mentioned above. The idea was to grow acidogenic and methanogenic bacteria in separate reactors so that the effect of MoO4 2- could be evaluated independently. The experiment comprised a 4.55-l packed-bed acidogenic reactor and a 4.75-l suspended-biomass methanogenic reactor operated over a period of more that 3 months. Glucose and Na2SO4 were used as the carbon and sulphate sources respectively. The concentration of MoO4 2- used in the test was 2.5 mM. The study showed that MoO4 2- inhibited both sulphate reduction as well as methane production and caused a change in VFA dominance from acetic to butyric acid. Acetic acid dominance in the reactor was resumed after the loading rate was decreased. Whereas SRBs showed complete recovery from the MoO4 2- dose once it was omitted from the feed, MPBs did not recover. MoO4 2- was bacteriostatic to SRBs and bacteriocidal to MPBs. Acidogenic bacteria were the first to acclimatise to MoO4 2-. MoO4 2- is not a suitable selective inhibitor of SRBs in anaerobic reactors. 2005 Article PeerReviewed application/pdf http://eprints.utp.edu.my/1280/1/Molybdate_inhibition_of_sulphate_reduction_in_two-phase_anaerobic_digestion.pdf Isa, M.H. and Anderson, G.K. (2005) Molybdate inhibition of sulphate reduction in two-phase anaerobic digestion. Process Biochemistry, 40 (6). pp. 2079-2089. http://eprints.utp.edu.my/1280/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
topic TD Environmental technology. Sanitary engineering
spellingShingle TD Environmental technology. Sanitary engineering
Isa, M.H.
Anderson, G.K.
Molybdate inhibition of sulphate reduction in two-phase anaerobic digestion
description Several researchers have used molybdate (MoO4 2-) for the selective inhibition of sulphate reducing bacteria (SRB) in anaerobic digestion. The feasibility of using MoO4 2- for the control of sulphate reduction in biological reactors, however, is not well established as some published results have also indicated an adverse effect on methane producing bacteria (MPB). Two possible reasons may be attributed for this observation, namely: (a) chronic inhibition of MPBs by MoO4 2- and (b) preferential acclimatisation of acidogenic bacteria to MoO4 2-. A two-phase anaerobic digestion experiment was conducted to establish the reason for the cessation of methanogenesis as mentioned above. The idea was to grow acidogenic and methanogenic bacteria in separate reactors so that the effect of MoO4 2- could be evaluated independently. The experiment comprised a 4.55-l packed-bed acidogenic reactor and a 4.75-l suspended-biomass methanogenic reactor operated over a period of more that 3 months. Glucose and Na2SO4 were used as the carbon and sulphate sources respectively. The concentration of MoO4 2- used in the test was 2.5 mM. The study showed that MoO4 2- inhibited both sulphate reduction as well as methane production and caused a change in VFA dominance from acetic to butyric acid. Acetic acid dominance in the reactor was resumed after the loading rate was decreased. Whereas SRBs showed complete recovery from the MoO4 2- dose once it was omitted from the feed, MPBs did not recover. MoO4 2- was bacteriostatic to SRBs and bacteriocidal to MPBs. Acidogenic bacteria were the first to acclimatise to MoO4 2-. MoO4 2- is not a suitable selective inhibitor of SRBs in anaerobic reactors.
format Article
author Isa, M.H.
Anderson, G.K.
author_sort Isa, M.H.
title Molybdate inhibition of sulphate reduction in two-phase anaerobic digestion
title_short Molybdate inhibition of sulphate reduction in two-phase anaerobic digestion
title_full Molybdate inhibition of sulphate reduction in two-phase anaerobic digestion
title_fullStr Molybdate inhibition of sulphate reduction in two-phase anaerobic digestion
title_full_unstemmed Molybdate inhibition of sulphate reduction in two-phase anaerobic digestion
title_sort molybdate inhibition of sulphate reduction in two-phase anaerobic digestion
publishDate 2005
url http://eprints.utp.edu.my/1280/1/Molybdate_inhibition_of_sulphate_reduction_in_two-phase_anaerobic_digestion.pdf
http://eprints.utp.edu.my/1280/
_version_ 1741196049521836032
score 11.62408

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