Aptamer-based determination of ATP by using a functionalized impedimetric nanosensor and mediation by a triangular junction transducer

The authors describe an impedimetric nanosensor for aptamer-mediated detection of ATP. A triangular junction of polysilicon substrate is used as a transducer. The aptamer was immobilized on the substrate via biotin-streptavidin interaction. ATP can be detected by this method in the 10 to 100 nM conc...

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Main Authors: Gopinath, S.C.B., Perumal, V., Balakrishnan, S.R., Md Arshad, M.K., Lakshmipriya, T., Haarindraprasad, R., Hashim, U.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.19301 /
Published: Springer-Verlag Wien 2017
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028824303&doi=10.1007%2fs00604-017-2485-8&partnerID=40&md5=dbec50d8a23b973c1304c4d9881b80cf
http://eprints.utp.edu.my/19301/
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spelling utp-eprints.193012018-05-03T02:10:27Z Aptamer-based determination of ATP by using a functionalized impedimetric nanosensor and mediation by a triangular junction transducer Gopinath, S.C.B. Perumal, V. Balakrishnan, S.R. Md Arshad, M.K. Lakshmipriya, T. Haarindraprasad, R. Hashim, U. The authors describe an impedimetric nanosensor for aptamer-mediated detection of ATP. A triangular junction of polysilicon substrate is used as a transducer. The aptamer was immobilized on the substrate via biotin-streptavidin interaction. ATP can be detected by this method in the 10 to 100 nM concentration range at room temperature and estimated the signal to noise ratio above the range of 3σ. It was applied to the detection of ATP in 1:1000 diluted human urine. The desired transducer is highly reproducible and specific. It is selective over closely related molecules such as guanosine triphosphate, cytidine triphosphate and uridine triphosphate. The method is perceived to represent a valuable tool for the diagnosis for infections and inflammations. The novelties shown in this study compared to other sensors are, consumption of low sample volume (~ 1 μl), non-invasive and suitable with AC and DC currents. Figure not available: see fulltext.. © 2017, Springer-Verlag GmbH Austria. Springer-Verlag Wien 2017 Article PeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028824303&doi=10.1007%2fs00604-017-2485-8&partnerID=40&md5=dbec50d8a23b973c1304c4d9881b80cf Gopinath, S.C.B. and Perumal, V. and Balakrishnan, S.R. and Md Arshad, M.K. and Lakshmipriya, T. and Haarindraprasad, R. and Hashim, U. (2017) Aptamer-based determination of ATP by using a functionalized impedimetric nanosensor and mediation by a triangular junction transducer. Microchimica Acta, 184 (11). pp. 4425-4431. http://eprints.utp.edu.my/19301/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description The authors describe an impedimetric nanosensor for aptamer-mediated detection of ATP. A triangular junction of polysilicon substrate is used as a transducer. The aptamer was immobilized on the substrate via biotin-streptavidin interaction. ATP can be detected by this method in the 10 to 100 nM concentration range at room temperature and estimated the signal to noise ratio above the range of 3σ. It was applied to the detection of ATP in 1:1000 diluted human urine. The desired transducer is highly reproducible and specific. It is selective over closely related molecules such as guanosine triphosphate, cytidine triphosphate and uridine triphosphate. The method is perceived to represent a valuable tool for the diagnosis for infections and inflammations. The novelties shown in this study compared to other sensors are, consumption of low sample volume (~ 1 μl), non-invasive and suitable with AC and DC currents. Figure not available: see fulltext.. © 2017, Springer-Verlag GmbH Austria.
format Article
author Gopinath, S.C.B.
Perumal, V.
Balakrishnan, S.R.
Md Arshad, M.K.
Lakshmipriya, T.
Haarindraprasad, R.
Hashim, U.
spellingShingle Gopinath, S.C.B.
Perumal, V.
Balakrishnan, S.R.
Md Arshad, M.K.
Lakshmipriya, T.
Haarindraprasad, R.
Hashim, U.
Aptamer-based determination of ATP by using a functionalized impedimetric nanosensor and mediation by a triangular junction transducer
author_sort Gopinath, S.C.B.
title Aptamer-based determination of ATP by using a functionalized impedimetric nanosensor and mediation by a triangular junction transducer
title_short Aptamer-based determination of ATP by using a functionalized impedimetric nanosensor and mediation by a triangular junction transducer
title_full Aptamer-based determination of ATP by using a functionalized impedimetric nanosensor and mediation by a triangular junction transducer
title_fullStr Aptamer-based determination of ATP by using a functionalized impedimetric nanosensor and mediation by a triangular junction transducer
title_full_unstemmed Aptamer-based determination of ATP by using a functionalized impedimetric nanosensor and mediation by a triangular junction transducer
title_sort aptamer-based determination of atp by using a functionalized impedimetric nanosensor and mediation by a triangular junction transducer
publisher Springer-Verlag Wien
publishDate 2017
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028824303&doi=10.1007%2fs00604-017-2485-8&partnerID=40&md5=dbec50d8a23b973c1304c4d9881b80cf
http://eprints.utp.edu.my/19301/
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score 11.62408

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