Ocular Blood Flow in Rabbits under Deep Anesthesia: A Real-Time Measurement Technique and Its Application in Characterizing Retinal Ischemia

In this study, we reported a new technique based on laser speckle flowgraphy to record the ocular blood flow in rabbits under deep anesthesia, and proposed parameters to characterize retinal ischemia. We applied the proposed technique to study the correlation of blood flow between the eyes of normal...

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Main Authors: Bhatti, M.S., Tang, T.B., Chen, H.C.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.20738 /
Published: Nature Publishing Group 2018
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045198223&doi=10.1038%2fs41598-018-24141-4&partnerID=40&md5=8ad3d0df23d35ec64707e10d36356c50
http://eprints.utp.edu.my/20738/
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spelling utp-eprints.207382019-02-26T02:22:42Z Ocular Blood Flow in Rabbits under Deep Anesthesia: A Real-Time Measurement Technique and Its Application in Characterizing Retinal Ischemia Bhatti, M.S. Tang, T.B. Chen, H.C. In this study, we reported a new technique based on laser speckle flowgraphy to record the ocular blood flow in rabbits under deep anesthesia, and proposed parameters to characterize retinal ischemia. We applied the proposed technique to study the correlation of blood flow between the eyes of normal non-anesthetized animals, and to characterize the occlusion of the internal carotid artery (ICA) and external carotid artery (ECA). We established a correlation in blood flow between the eyes of non-anesthetized animals, and derived two new parameters, namely, the laterality index and vascular perfusion estimate (VPE). Our experimental results from 16 eyes (of 13 New Zealand white rabbits) showed a reduction in ocular blood flow with a significant decrease in the VPE after the occlusion of the ECA (p < 0.001). A low/minimal effect on blood flow was observed with the occlusion of the ICA. In conclusion, we demonstrated a means for the real-time measurement of the ocular blood flow in rabbits under deep anesthesia by using laser speckle flowgraphy and the VPE as an indicator of successful occlusion. The proposed technique might be applicable in quantifying the efficacy of new drugs and interventions for the treatment of retinal ischemia. © 2018 The Author(s). Nature Publishing Group 2018 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045198223&doi=10.1038%2fs41598-018-24141-4&partnerID=40&md5=8ad3d0df23d35ec64707e10d36356c50 Bhatti, M.S. and Tang, T.B. and Chen, H.C. (2018) Ocular Blood Flow in Rabbits under Deep Anesthesia: A Real-Time Measurement Technique and Its Application in Characterizing Retinal Ischemia. Scientific Reports, 8 (1). http://eprints.utp.edu.my/20738/
institution Universiti Teknologi Petronas
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description In this study, we reported a new technique based on laser speckle flowgraphy to record the ocular blood flow in rabbits under deep anesthesia, and proposed parameters to characterize retinal ischemia. We applied the proposed technique to study the correlation of blood flow between the eyes of normal non-anesthetized animals, and to characterize the occlusion of the internal carotid artery (ICA) and external carotid artery (ECA). We established a correlation in blood flow between the eyes of non-anesthetized animals, and derived two new parameters, namely, the laterality index and vascular perfusion estimate (VPE). Our experimental results from 16 eyes (of 13 New Zealand white rabbits) showed a reduction in ocular blood flow with a significant decrease in the VPE after the occlusion of the ECA (p < 0.001). A low/minimal effect on blood flow was observed with the occlusion of the ICA. In conclusion, we demonstrated a means for the real-time measurement of the ocular blood flow in rabbits under deep anesthesia by using laser speckle flowgraphy and the VPE as an indicator of successful occlusion. The proposed technique might be applicable in quantifying the efficacy of new drugs and interventions for the treatment of retinal ischemia. © 2018 The Author(s).
format Article
author Bhatti, M.S.
Tang, T.B.
Chen, H.C.
spellingShingle Bhatti, M.S.
Tang, T.B.
Chen, H.C.
Ocular Blood Flow in Rabbits under Deep Anesthesia: A Real-Time Measurement Technique and Its Application in Characterizing Retinal Ischemia
author_sort Bhatti, M.S.
title Ocular Blood Flow in Rabbits under Deep Anesthesia: A Real-Time Measurement Technique and Its Application in Characterizing Retinal Ischemia
title_short Ocular Blood Flow in Rabbits under Deep Anesthesia: A Real-Time Measurement Technique and Its Application in Characterizing Retinal Ischemia
title_full Ocular Blood Flow in Rabbits under Deep Anesthesia: A Real-Time Measurement Technique and Its Application in Characterizing Retinal Ischemia
title_fullStr Ocular Blood Flow in Rabbits under Deep Anesthesia: A Real-Time Measurement Technique and Its Application in Characterizing Retinal Ischemia
title_full_unstemmed Ocular Blood Flow in Rabbits under Deep Anesthesia: A Real-Time Measurement Technique and Its Application in Characterizing Retinal Ischemia
title_sort ocular blood flow in rabbits under deep anesthesia: a real-time measurement technique and its application in characterizing retinal ischemia
publisher Nature Publishing Group
publishDate 2018
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045198223&doi=10.1038%2fs41598-018-24141-4&partnerID=40&md5=8ad3d0df23d35ec64707e10d36356c50
http://eprints.utp.edu.my/20738/
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score 11.62408

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