Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis

Tuberculosis (TB) is a chronic and infectious airborne disease which requires a diagnosing system with high sensitivity and specificity. However, the traditional gold standard method for TB detection remains unreliable with low specificity and sensitivity. Nanostructured composite materials coupled...

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Main Authors: Perumal, V., Saheed, M.S.M., Mohamed, N.M., Murthe, S.S., Gopinath, S.C.B., Chiu, J.-M.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.21410 /
Published: Elsevier Ltd 2018
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048483510&doi=10.1016%2fj.bios.2018.05.042&partnerID=40&md5=5b7def20a74217dd1f3b99af5ad8ff32
http://eprints.utp.edu.my/21410/
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spelling utp-eprints.214102019-01-10T05:31:24Z Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis Perumal, V. Saheed, M.S.M. Mohamed, N.M. Saheed, M.S.M. Murthe, S.S. Gopinath, S.C.B. Chiu, J.-M. Tuberculosis (TB) is a chronic and infectious airborne disease which requires a diagnosing system with high sensitivity and specificity. However, the traditional gold standard method for TB detection remains unreliable with low specificity and sensitivity. Nanostructured composite materials coupled with impedimetric sensing utilised in this study offered a feasible solution. Herein, novel gold (Au) nanorods were synthesized on 3D graphene grown by chemical vapour deposition. The irregularly spaced and rippled morphology of 3D graphene provided a path for Au nanoparticles to self-assemble and form rod-like structures on the surface of the 3D graphene. The formation of Au nanorods were showcased through scanning electron microscopy which revealed the evolution of Au nanoparticle into Au islets. Eventually, it formed nanorods possessing lengths of ~ 150 nm and diameters of ~ 30 nm. The X-ray diffractogram displayed appropriate peaks suitable to defect-free and high crystalline graphene with face centered cubic Au. The strong optical interrelation between Au nanorod and 3D graphene was elucidated by Raman spectroscopy analysis. Furthermore, the anchored Au nanorods on 3D graphene nanocomposite enables feasible bio-capturing on the exposed Au surface on defect free graphene. The impedimetric sensing of DNA sequence from TB on 3D graphene/Au nanocomposite revealed a remarkable wide detection linear range from 10 fM to 0.1 µM, displays the capability of detecting femtomolar DNA concentration. Overall, the novel 3D graphene/Au nanocomposite demonstrated here offers high-performance bio-sensing and opens a new avenue for TB detection. © 2018 Elsevier B.V. Elsevier Ltd 2018 Article PeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048483510&doi=10.1016%2fj.bios.2018.05.042&partnerID=40&md5=5b7def20a74217dd1f3b99af5ad8ff32 Perumal, V. and Saheed, M.S.M. and Mohamed, N.M. and Saheed, M.S.M. and Murthe, S.S. and Gopinath, S.C.B. and Chiu, J.-M. (2018) Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis. Biosensors and Bioelectronics, 116 . pp. 116-122. http://eprints.utp.edu.my/21410/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description Tuberculosis (TB) is a chronic and infectious airborne disease which requires a diagnosing system with high sensitivity and specificity. However, the traditional gold standard method for TB detection remains unreliable with low specificity and sensitivity. Nanostructured composite materials coupled with impedimetric sensing utilised in this study offered a feasible solution. Herein, novel gold (Au) nanorods were synthesized on 3D graphene grown by chemical vapour deposition. The irregularly spaced and rippled morphology of 3D graphene provided a path for Au nanoparticles to self-assemble and form rod-like structures on the surface of the 3D graphene. The formation of Au nanorods were showcased through scanning electron microscopy which revealed the evolution of Au nanoparticle into Au islets. Eventually, it formed nanorods possessing lengths of ~ 150 nm and diameters of ~ 30 nm. The X-ray diffractogram displayed appropriate peaks suitable to defect-free and high crystalline graphene with face centered cubic Au. The strong optical interrelation between Au nanorod and 3D graphene was elucidated by Raman spectroscopy analysis. Furthermore, the anchored Au nanorods on 3D graphene nanocomposite enables feasible bio-capturing on the exposed Au surface on defect free graphene. The impedimetric sensing of DNA sequence from TB on 3D graphene/Au nanocomposite revealed a remarkable wide detection linear range from 10 fM to 0.1 µM, displays the capability of detecting femtomolar DNA concentration. Overall, the novel 3D graphene/Au nanocomposite demonstrated here offers high-performance bio-sensing and opens a new avenue for TB detection. © 2018 Elsevier B.V.
format Article
author Perumal, V.
Saheed, M.S.M.
Mohamed, N.M.
Saheed, M.S.M.
Murthe, S.S.
Gopinath, S.C.B.
Chiu, J.-M.
spellingShingle Perumal, V.
Saheed, M.S.M.
Mohamed, N.M.
Saheed, M.S.M.
Murthe, S.S.
Gopinath, S.C.B.
Chiu, J.-M.
Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis
author_sort Perumal, V.
title Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis
title_short Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis
title_full Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis
title_fullStr Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis
title_full_unstemmed Gold nanorod embedded novel 3D graphene nanocomposite for selective bio-capture in rapid detection of Mycobacterium tuberculosis
title_sort gold nanorod embedded novel 3d graphene nanocomposite for selective bio-capture in rapid detection of mycobacterium tuberculosis
publisher Elsevier Ltd
publishDate 2018
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048483510&doi=10.1016%2fj.bios.2018.05.042&partnerID=40&md5=5b7def20a74217dd1f3b99af5ad8ff32
http://eprints.utp.edu.my/21410/
_version_ 1741196455004078080
score 11.62408

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