Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature

Seeds of a renowned medicinal plant, giant dodder (Cuscuta reflexa), were assessed to appraise the effect of solvent and extraction technique variation on antioxidants potential. Dodder seed, also called cuscuta seed, has been considered superb tonic in traditional herbal medication for eyes, liver,...

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Main Authors: Noureen, S., Noreen, S., Ghumman, S.A., Batool, F., Arshad, M., Noreen, F., Ishtiaq, U., Bukhari, S.N.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.22022 /
Published: Academic Press 2018
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047661197&doi=10.15835%2fnbha46211088&partnerID=40&md5=403e13918ac6db76cc3606a3f8bdc714
http://eprints.utp.edu.my/22022/
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spelling utp-eprints.220222018-08-01T01:01:28Z Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature Noureen, S. Noreen, S. Ghumman, S.A. Batool, F. Arshad, M. Noreen, F. Ishtiaq, U. Bukhari, S.N. Seeds of a renowned medicinal plant, giant dodder (Cuscuta reflexa), were assessed to appraise the effect of solvent and extraction technique variation on antioxidants potential. Dodder seed, also called cuscuta seed, has been considered superb tonic in traditional herbal medication for eyes, liver, spleen and kidney. Results show that selected solvent and procedure plays a key role in the composition and activity of extractable material. Three extraction procedures Orbital shaker, Decoction and Ultrasonic assisted extraction and five different solvents n-hexane, ethyl acetate, 100 methanol, 80 methanol and 60 methanol were used to get optimized conditions. Total phenolic and flavonoids content were found maximum in the extracts of aqueous organic system containing 80 methanol in Ultrasonic assisted extraction method but in case of tannins ethyl acetate and Orbital shaker extraction was found more suitable partner. Antioxidant estimation assays showed a little bit variation as DPPH and ABTS exhibited maximum inhibition in 80 methanol and Ultrasonic assisted extraction but 100 methanol was found better for FRAP assay. Decoction results were mostly in between the both Orbital shaker and Ultrasonic assisted extraction. Overall results indicate that coexistence of polar solvents and Ultrasonic assisted extraction gives a better choice for extractability of potent antioxidants from seeds. HPLC analysis confirmed presence of valuable phenolic acids. Pearson's correlation coefficient reveals a significant relationship between extracted components and antioxidant capacity P < 0.05 or 0.01. © 2018 Academic Press. Academic Press 2018 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047661197&doi=10.15835%2fnbha46211088&partnerID=40&md5=403e13918ac6db76cc3606a3f8bdc714 Noureen, S. and Noreen, S. and Ghumman, S.A. and Batool, F. and Arshad, M. and Noreen, F. and Ishtiaq, U. and Bukhari, S.N. (2018) Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46 (2). pp. 653-662. http://eprints.utp.edu.my/22022/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description Seeds of a renowned medicinal plant, giant dodder (Cuscuta reflexa), were assessed to appraise the effect of solvent and extraction technique variation on antioxidants potential. Dodder seed, also called cuscuta seed, has been considered superb tonic in traditional herbal medication for eyes, liver, spleen and kidney. Results show that selected solvent and procedure plays a key role in the composition and activity of extractable material. Three extraction procedures Orbital shaker, Decoction and Ultrasonic assisted extraction and five different solvents n-hexane, ethyl acetate, 100 methanol, 80 methanol and 60 methanol were used to get optimized conditions. Total phenolic and flavonoids content were found maximum in the extracts of aqueous organic system containing 80 methanol in Ultrasonic assisted extraction method but in case of tannins ethyl acetate and Orbital shaker extraction was found more suitable partner. Antioxidant estimation assays showed a little bit variation as DPPH and ABTS exhibited maximum inhibition in 80 methanol and Ultrasonic assisted extraction but 100 methanol was found better for FRAP assay. Decoction results were mostly in between the both Orbital shaker and Ultrasonic assisted extraction. Overall results indicate that coexistence of polar solvents and Ultrasonic assisted extraction gives a better choice for extractability of potent antioxidants from seeds. HPLC analysis confirmed presence of valuable phenolic acids. Pearson's correlation coefficient reveals a significant relationship between extracted components and antioxidant capacity P < 0.05 or 0.01. © 2018 Academic Press.
format Article
author Noureen, S.
Noreen, S.
Ghumman, S.A.
Batool, F.
Arshad, M.
Noreen, F.
Ishtiaq, U.
Bukhari, S.N.
spellingShingle Noureen, S.
Noreen, S.
Ghumman, S.A.
Batool, F.
Arshad, M.
Noreen, F.
Ishtiaq, U.
Bukhari, S.N.
Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature
author_sort Noureen, S.
title Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature
title_short Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature
title_full Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature
title_fullStr Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature
title_full_unstemmed Seeds of giant dodder (Cuscuta reflexa) as a function of extract procedure and solvent nature
title_sort seeds of giant dodder (cuscuta reflexa) as a function of extract procedure and solvent nature
publisher Academic Press
publishDate 2018
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047661197&doi=10.15835%2fnbha46211088&partnerID=40&md5=403e13918ac6db76cc3606a3f8bdc714
http://eprints.utp.edu.my/22022/
_version_ 1741196559233581056
score 11.62408

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