Bioethanol Production from Musambi Peel by Acid Catalyzed Steam Pretreatment and Enzymatic Saccharification: Optimization of Delignification Using Taguchi Design

Abstract: Citrus waste is an attractive feedstock for second generation bioethanol due to its richness in carbohydrates. For the production of bioethanol, removal of lignin through pretreatment to liberate more cellulose with minimal loss is essential. In the present work, the removal of lignin from...

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Main Authors: John, I., Pola, J., Thanabalan, M., Appusamy, A.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.22230 /
Published: 2019
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060132098&doi=10.1007%2fs12649-018-00565-x&partnerID=40&md5=cb9cacb718bd8847fc100bbb80416929
http://eprints.utp.edu.my/22230/
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spelling utp-eprints.222302019-02-28T02:51:40Z Bioethanol Production from Musambi Peel by Acid Catalyzed Steam Pretreatment and Enzymatic Saccharification: Optimization of Delignification Using Taguchi Design John, I. Pola, J. Thanabalan, M. Appusamy, A. Abstract: Citrus waste is an attractive feedstock for second generation bioethanol due to its richness in carbohydrates. For the production of bioethanol, removal of lignin through pretreatment to liberate more cellulose with minimal loss is essential. In the present work, the removal of lignin from musambi (Citrus limetta) peel by acid-catalyzed steam pretreatment and the characterization of musambi peel before and after pretreatment was studied. A Taguchi design was used to optimize solid loading, sulphuric acid concentration and time, for steam pretreatment with delignification as the response. The optimum conditions of process variables were 17 (w/v) for solid loading, 0.25 (v/v) for acid concentration with a time of exposure of 60 min with maximum delignification of 65. The decrease in peak intensity in FTIR spectra and increase in the crystallinity index of pretreated peel indicated the reduction in pectin, hemicellulose, and lignin. The scanning electron micrographs of pretreated peel clearly showed the delignified microfibrils of cellulose. Enzymatic saccharification of pretreated musambi peel and the effect of peel loading, temperature, time, pH and the loading of cellulase, beta-glucosidase, and pectinase on reducing sugar were also investigated. The maximum reducing sugar obtained after the enzymatic hydrolysis was 386 mg/g for steam pretreated musambi peel at the optimum conditions whereas it was only 258 mg/g for the raw musambi peel. Thus, steam pretreatment of musambi peel resulted in better hydrolysis than untreated musambi peel. The yield of bioethanol production at the end of 48 h of fermentation was 85.97. © 2019, Springer Nature B.V. 2019 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060132098&doi=10.1007%2fs12649-018-00565-x&partnerID=40&md5=cb9cacb718bd8847fc100bbb80416929 John, I. and Pola, J. and Thanabalan, M. and Appusamy, A. (2019) Bioethanol Production from Musambi Peel by Acid Catalyzed Steam Pretreatment and Enzymatic Saccharification: Optimization of Delignification Using Taguchi Design. Waste and Biomass Valorization . http://eprints.utp.edu.my/22230/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description Abstract: Citrus waste is an attractive feedstock for second generation bioethanol due to its richness in carbohydrates. For the production of bioethanol, removal of lignin through pretreatment to liberate more cellulose with minimal loss is essential. In the present work, the removal of lignin from musambi (Citrus limetta) peel by acid-catalyzed steam pretreatment and the characterization of musambi peel before and after pretreatment was studied. A Taguchi design was used to optimize solid loading, sulphuric acid concentration and time, for steam pretreatment with delignification as the response. The optimum conditions of process variables were 17 (w/v) for solid loading, 0.25 (v/v) for acid concentration with a time of exposure of 60 min with maximum delignification of 65. The decrease in peak intensity in FTIR spectra and increase in the crystallinity index of pretreated peel indicated the reduction in pectin, hemicellulose, and lignin. The scanning electron micrographs of pretreated peel clearly showed the delignified microfibrils of cellulose. Enzymatic saccharification of pretreated musambi peel and the effect of peel loading, temperature, time, pH and the loading of cellulase, beta-glucosidase, and pectinase on reducing sugar were also investigated. The maximum reducing sugar obtained after the enzymatic hydrolysis was 386 mg/g for steam pretreated musambi peel at the optimum conditions whereas it was only 258 mg/g for the raw musambi peel. Thus, steam pretreatment of musambi peel resulted in better hydrolysis than untreated musambi peel. The yield of bioethanol production at the end of 48 h of fermentation was 85.97. © 2019, Springer Nature B.V.
format Article
author John, I.
Pola, J.
Thanabalan, M.
Appusamy, A.
spellingShingle John, I.
Pola, J.
Thanabalan, M.
Appusamy, A.
Bioethanol Production from Musambi Peel by Acid Catalyzed Steam Pretreatment and Enzymatic Saccharification: Optimization of Delignification Using Taguchi Design
author_sort John, I.
title Bioethanol Production from Musambi Peel by Acid Catalyzed Steam Pretreatment and Enzymatic Saccharification: Optimization of Delignification Using Taguchi Design
title_short Bioethanol Production from Musambi Peel by Acid Catalyzed Steam Pretreatment and Enzymatic Saccharification: Optimization of Delignification Using Taguchi Design
title_full Bioethanol Production from Musambi Peel by Acid Catalyzed Steam Pretreatment and Enzymatic Saccharification: Optimization of Delignification Using Taguchi Design
title_fullStr Bioethanol Production from Musambi Peel by Acid Catalyzed Steam Pretreatment and Enzymatic Saccharification: Optimization of Delignification Using Taguchi Design
title_full_unstemmed Bioethanol Production from Musambi Peel by Acid Catalyzed Steam Pretreatment and Enzymatic Saccharification: Optimization of Delignification Using Taguchi Design
title_sort bioethanol production from musambi peel by acid catalyzed steam pretreatment and enzymatic saccharification: optimization of delignification using taguchi design
publishDate 2019
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060132098&doi=10.1007%2fs12649-018-00565-x&partnerID=40&md5=cb9cacb718bd8847fc100bbb80416929
http://eprints.utp.edu.my/22230/
_version_ 1741196588658720768
score 11.62408

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