Lipase immobilization on fibers grafted with polyglycidyl methachrylate

Lipase enzyme originated from wheat germ was immobilized on nylon -6- grafted with polyglycidyl methachrylate (PGMA). The immobilization of enzyme experiments were designed and studied using face centred central composite design (FCCCD) under response surface methodology (RSM). Prior to immobilizati...

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Main Authors: Alkhatib, M.F.R., Bahrudin, N.A., Salleh, H.M., Nasef, M.M.E., Ting, T.M.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.22865 /
Published: International Islamic University Malaysia-IIUM 2019
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067368060&doi=10.31436%2fiiumej.v20i1.1002&partnerID=40&md5=342e6f1a2c048b10a29fe62e84b8e474
http://eprints.utp.edu.my/22865/
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spelling utp-eprints.228652019-07-08T06:29:16Z Lipase immobilization on fibers grafted with polyglycidyl methachrylate Alkhatib, M.F.R. Bahrudin, N.A. Salleh, H.M. Nasef, M.M.E. Ting, T.M. Lipase enzyme originated from wheat germ was immobilized on nylon -6- grafted with polyglycidyl methachrylate (PGMA). The immobilization of enzyme experiments were designed and studied using face centred central composite design (FCCCD) under response surface methodology (RSM). Prior to immobilization, the polymer was activated with diethyl amine/ethanol to introduce an amine functional group to facilitate covalent bonding with the enzyme. The immobilized and free enzymes were characterized for effect of temperature and pH on enzyme activity, stability, storage and reusability as well as kinetics studies. ANOVA revealed that optimum lipase activity of 0.287 U/ml was achieved at immobilization time of 5 h, pH of 6 and 1.0 mg/ml for enzyme concentration. The optimum temperatures and pH for immobilized and free enzymes were 45 °C and 35 °C, and 8 and 7, respectively. The immobilized enzyme showed higher stability compared to free enzyme. The immobilized enzyme retained 18 of its activity after being recycled 8 times. In a storage stability test, immobilized lipase was able to retain 70 of its activity after being stored for 30 days, while free enzyme activity dropped to 15 after 20 days of storage. © 2019, International Islamic University Malaysia-IIUM. International Islamic University Malaysia-IIUM 2019 Article PeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067368060&doi=10.31436%2fiiumej.v20i1.1002&partnerID=40&md5=342e6f1a2c048b10a29fe62e84b8e474 Alkhatib, M.F.R. and Bahrudin, N.A. and Salleh, H.M. and Nasef, M.M.E. and Ting, T.M. (2019) Lipase immobilization on fibers grafted with polyglycidyl methachrylate. IIUM Engineering Journal, 20 (1). pp. 12-23. http://eprints.utp.edu.my/22865/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description Lipase enzyme originated from wheat germ was immobilized on nylon -6- grafted with polyglycidyl methachrylate (PGMA). The immobilization of enzyme experiments were designed and studied using face centred central composite design (FCCCD) under response surface methodology (RSM). Prior to immobilization, the polymer was activated with diethyl amine/ethanol to introduce an amine functional group to facilitate covalent bonding with the enzyme. The immobilized and free enzymes were characterized for effect of temperature and pH on enzyme activity, stability, storage and reusability as well as kinetics studies. ANOVA revealed that optimum lipase activity of 0.287 U/ml was achieved at immobilization time of 5 h, pH of 6 and 1.0 mg/ml for enzyme concentration. The optimum temperatures and pH for immobilized and free enzymes were 45 °C and 35 °C, and 8 and 7, respectively. The immobilized enzyme showed higher stability compared to free enzyme. The immobilized enzyme retained 18 of its activity after being recycled 8 times. In a storage stability test, immobilized lipase was able to retain 70 of its activity after being stored for 30 days, while free enzyme activity dropped to 15 after 20 days of storage. © 2019, International Islamic University Malaysia-IIUM.
format Article
author Alkhatib, M.F.R.
Bahrudin, N.A.
Salleh, H.M.
Nasef, M.M.E.
Ting, T.M.
spellingShingle Alkhatib, M.F.R.
Bahrudin, N.A.
Salleh, H.M.
Nasef, M.M.E.
Ting, T.M.
Lipase immobilization on fibers grafted with polyglycidyl methachrylate
author_sort Alkhatib, M.F.R.
title Lipase immobilization on fibers grafted with polyglycidyl methachrylate
title_short Lipase immobilization on fibers grafted with polyglycidyl methachrylate
title_full Lipase immobilization on fibers grafted with polyglycidyl methachrylate
title_fullStr Lipase immobilization on fibers grafted with polyglycidyl methachrylate
title_full_unstemmed Lipase immobilization on fibers grafted with polyglycidyl methachrylate
title_sort lipase immobilization on fibers grafted with polyglycidyl methachrylate
publisher International Islamic University Malaysia-IIUM
publishDate 2019
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067368060&doi=10.31436%2fiiumej.v20i1.1002&partnerID=40&md5=342e6f1a2c048b10a29fe62e84b8e474
http://eprints.utp.edu.my/22865/
_version_ 1741196604270968832
score 11.62408

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