Label-free, high-throughput assay of human dendritic cells from whole-blood samples with microfluidic inertial separation suitable for resource-limited manufacturing

Microfluidics technology has not impacted the delivery and accessibility of point-of-care health services, like diagnosing infectious disease, monitoring health or delivering interventions. Most microfluidics prototypes in academic research are not easy to scale-up with industrial-scale fabrication...

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Main Authors: Caffiyar, M.Y., Lim, K.P., Kamal Basha, I.H., Hamid, N.H., Cheong, S.C., Wei Ho, E.T.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.23348 /
Published: MDPI AG 2020
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085948956&doi=10.3390%2fmi11050514&partnerID=40&md5=899621bba859a70cc19f906582dee2ad
http://eprints.utp.edu.my/23348/
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spelling utp-eprints.233482021-08-19T07:24:05Z Label-free, high-throughput assay of human dendritic cells from whole-blood samples with microfluidic inertial separation suitable for resource-limited manufacturing Caffiyar, M.Y. Lim, K.P. Kamal Basha, I.H. Hamid, N.H. Cheong, S.C. Wei Ho, E.T. Microfluidics technology has not impacted the delivery and accessibility of point-of-care health services, like diagnosing infectious disease, monitoring health or delivering interventions. Most microfluidics prototypes in academic research are not easy to scale-up with industrial-scale fabrication techniques and cannot be operated without complex manipulations of supporting equipment and additives, such as labels or reagents. We propose a label-and reagent-free inertial spiral microfluidic device to separate red blood, white blood and dendritic cells from blood fluid, for applications in health monitoring and immunotherapy. We demonstrate that using larger channel widths, in the range of 200 to 600 μm, allows separation of cells into multiple focused streams, according to different size ranges, and we utilize a novel technique to collect the closely separated focused cell streams, without constricting the channel. Our contribution is a method to adapt spiral inertial microfluidic designs to separate more than two cell types in the same device, which is robust against clogging, simple to operate and suitable for fabrication and deployment in resource-limited populations. When tested on actual human blood cells, 77 of dendritic cells were separated and 80 of cells remained viable after our assay. © 2020 by the authors. MDPI AG 2020 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085948956&doi=10.3390%2fmi11050514&partnerID=40&md5=899621bba859a70cc19f906582dee2ad Caffiyar, M.Y. and Lim, K.P. and Kamal Basha, I.H. and Hamid, N.H. and Cheong, S.C. and Wei Ho, E.T. (2020) Label-free, high-throughput assay of human dendritic cells from whole-blood samples with microfluidic inertial separation suitable for resource-limited manufacturing. Micromachines, 11 (5). http://eprints.utp.edu.my/23348/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description Microfluidics technology has not impacted the delivery and accessibility of point-of-care health services, like diagnosing infectious disease, monitoring health or delivering interventions. Most microfluidics prototypes in academic research are not easy to scale-up with industrial-scale fabrication techniques and cannot be operated without complex manipulations of supporting equipment and additives, such as labels or reagents. We propose a label-and reagent-free inertial spiral microfluidic device to separate red blood, white blood and dendritic cells from blood fluid, for applications in health monitoring and immunotherapy. We demonstrate that using larger channel widths, in the range of 200 to 600 μm, allows separation of cells into multiple focused streams, according to different size ranges, and we utilize a novel technique to collect the closely separated focused cell streams, without constricting the channel. Our contribution is a method to adapt spiral inertial microfluidic designs to separate more than two cell types in the same device, which is robust against clogging, simple to operate and suitable for fabrication and deployment in resource-limited populations. When tested on actual human blood cells, 77 of dendritic cells were separated and 80 of cells remained viable after our assay. © 2020 by the authors.
format Article
author Caffiyar, M.Y.
Lim, K.P.
Kamal Basha, I.H.
Hamid, N.H.
Cheong, S.C.
Wei Ho, E.T.
spellingShingle Caffiyar, M.Y.
Lim, K.P.
Kamal Basha, I.H.
Hamid, N.H.
Cheong, S.C.
Wei Ho, E.T.
Label-free, high-throughput assay of human dendritic cells from whole-blood samples with microfluidic inertial separation suitable for resource-limited manufacturing
author_sort Caffiyar, M.Y.
title Label-free, high-throughput assay of human dendritic cells from whole-blood samples with microfluidic inertial separation suitable for resource-limited manufacturing
title_short Label-free, high-throughput assay of human dendritic cells from whole-blood samples with microfluidic inertial separation suitable for resource-limited manufacturing
title_full Label-free, high-throughput assay of human dendritic cells from whole-blood samples with microfluidic inertial separation suitable for resource-limited manufacturing
title_fullStr Label-free, high-throughput assay of human dendritic cells from whole-blood samples with microfluidic inertial separation suitable for resource-limited manufacturing
title_full_unstemmed Label-free, high-throughput assay of human dendritic cells from whole-blood samples with microfluidic inertial separation suitable for resource-limited manufacturing
title_sort label-free, high-throughput assay of human dendritic cells from whole-blood samples with microfluidic inertial separation suitable for resource-limited manufacturing
publisher MDPI AG
publishDate 2020
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085948956&doi=10.3390%2fmi11050514&partnerID=40&md5=899621bba859a70cc19f906582dee2ad
http://eprints.utp.edu.my/23348/
_version_ 1741196661433040896
score 11.62408

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