Highly sensitive and selective acute myocardial infarction detection using aptamer-tethered MoS2 nanoflower and screen-printed electrodes

Acute myocardial infarction (AMI) is one of the leading causes of death worldwide. Cardiac troponin I (cTn1) is a commonly used biomarker for the diagnosis of AMI. Although there are various detection methods for the rapid detection of cTn1 such as optical, electrochemical, and acoustic techniques,...

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Main Authors: Vasudevan, M., Tai, M.J.Y., Perumal, V., Gopinath, S.C.B., Murthe, S.S., Ovinis, M., Mohamed, N.M., Joshi, N.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.23402 /
Published: Blackwell Publishing Ltd 2020
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096724439&doi=10.1002%2fbab.2060&partnerID=40&md5=d564927279aac1c5f5beff7df0e3d3e3
http://eprints.utp.edu.my/23402/
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spelling utp-eprints.234022021-08-19T07:22:28Z Highly sensitive and selective acute myocardial infarction detection using aptamer-tethered MoS2 nanoflower and screen-printed electrodes Vasudevan, M. Tai, M.J.Y. Perumal, V. Gopinath, S.C.B. Murthe, S.S. Ovinis, M. Mohamed, N.M. Joshi, N. Acute myocardial infarction (AMI) is one of the leading causes of death worldwide. Cardiac troponin I (cTn1) is a commonly used biomarker for the diagnosis of AMI. Although there are various detection methods for the rapid detection of cTn1 such as optical, electrochemical, and acoustic techniques, electrochemical aptasensing techniques are commonly used because of their ease of handling, portability, and compactness. In this study, an electrochemical cTn1 biosensor, MoS2 nanoflowers on screen-printed electrodes assisted by aptamer, was synthesized using hydrothermal technique. Field emission scanning electron microscopy revealed distinct 2D nanosheets and jagged flower-like 3D MoS2 nanoflower structure, with X-ray diffraction analysis revealing well-stacked MoS2layers. Voltammetry aptasensing of cTn1 ranges from 10 fM to 1 nM, with a detection limit at 10 fM and a sensitivity of 0.10 nA µM�1 cm�2. This is a �fivefold improvement in selectivity compared with the other proteins and human serum. This novel aptasensor retained 90 of its biosensing activity after 6 weeks with a 4.3 RSD and is a promising high-performance biosensor for detecting cTn1. © 2020 International Union of Biochemistry and Molecular Biology, Inc. Blackwell Publishing Ltd 2020 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096724439&doi=10.1002%2fbab.2060&partnerID=40&md5=d564927279aac1c5f5beff7df0e3d3e3 Vasudevan, M. and Tai, M.J.Y. and Perumal, V. and Gopinath, S.C.B. and Murthe, S.S. and Ovinis, M. and Mohamed, N.M. and Joshi, N. (2020) Highly sensitive and selective acute myocardial infarction detection using aptamer-tethered MoS2 nanoflower and screen-printed electrodes. Biotechnology and Applied Biochemistry . http://eprints.utp.edu.my/23402/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description Acute myocardial infarction (AMI) is one of the leading causes of death worldwide. Cardiac troponin I (cTn1) is a commonly used biomarker for the diagnosis of AMI. Although there are various detection methods for the rapid detection of cTn1 such as optical, electrochemical, and acoustic techniques, electrochemical aptasensing techniques are commonly used because of their ease of handling, portability, and compactness. In this study, an electrochemical cTn1 biosensor, MoS2 nanoflowers on screen-printed electrodes assisted by aptamer, was synthesized using hydrothermal technique. Field emission scanning electron microscopy revealed distinct 2D nanosheets and jagged flower-like 3D MoS2 nanoflower structure, with X-ray diffraction analysis revealing well-stacked MoS2layers. Voltammetry aptasensing of cTn1 ranges from 10 fM to 1 nM, with a detection limit at 10 fM and a sensitivity of 0.10 nA µM�1 cm�2. This is a �fivefold improvement in selectivity compared with the other proteins and human serum. This novel aptasensor retained 90 of its biosensing activity after 6 weeks with a 4.3 RSD and is a promising high-performance biosensor for detecting cTn1. © 2020 International Union of Biochemistry and Molecular Biology, Inc.
format Article
author Vasudevan, M.
Tai, M.J.Y.
Perumal, V.
Gopinath, S.C.B.
Murthe, S.S.
Ovinis, M.
Mohamed, N.M.
Joshi, N.
spellingShingle Vasudevan, M.
Tai, M.J.Y.
Perumal, V.
Gopinath, S.C.B.
Murthe, S.S.
Ovinis, M.
Mohamed, N.M.
Joshi, N.
Highly sensitive and selective acute myocardial infarction detection using aptamer-tethered MoS2 nanoflower and screen-printed electrodes
author_sort Vasudevan, M.
title Highly sensitive and selective acute myocardial infarction detection using aptamer-tethered MoS2 nanoflower and screen-printed electrodes
title_short Highly sensitive and selective acute myocardial infarction detection using aptamer-tethered MoS2 nanoflower and screen-printed electrodes
title_full Highly sensitive and selective acute myocardial infarction detection using aptamer-tethered MoS2 nanoflower and screen-printed electrodes
title_fullStr Highly sensitive and selective acute myocardial infarction detection using aptamer-tethered MoS2 nanoflower and screen-printed electrodes
title_full_unstemmed Highly sensitive and selective acute myocardial infarction detection using aptamer-tethered MoS2 nanoflower and screen-printed electrodes
title_sort highly sensitive and selective acute myocardial infarction detection using aptamer-tethered mos2 nanoflower and screen-printed electrodes
publisher Blackwell Publishing Ltd
publishDate 2020
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096724439&doi=10.1002%2fbab.2060&partnerID=40&md5=d564927279aac1c5f5beff7df0e3d3e3
http://eprints.utp.edu.my/23402/
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score 11.62408

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