Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor
Thyroid cancer appears in endocrine glands and specific to thyroid glands has been reported widely. This work was targeted to identify and quantify thyroglobulin by using antithyroglobulin antibody complexed silane surface on interdigitated electrode (IDE) sensing surface. (3-Aminopropyl)triethoxysi...
| Main Authors: | Wang, Y., Sun, X., Gopinath, S.C.B., Saheed, M.S.M., Wang, X. |
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| Format: | Article |
| Institution: | Universiti Teknologi Petronas |
| Record Id / ISBN-0: | utp-eprints.29024 / |
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John Wiley and Sons Inc
2022
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101627832&doi=10.1002%2fbab.2116&partnerID=40&md5=916cac84a6bc4a04f041e514cb98e942 http://eprints.utp.edu.my/29024/ |
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utp-eprints.290242022-03-17T16:39:32Z Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor Wang, Y. Sun, X. Gopinath, S.C.B. Saheed, M.S.M. Wang, X. Thyroid cancer appears in endocrine glands and specific to thyroid glands has been reported widely. This work was targeted to identify and quantify thyroglobulin by using antithyroglobulin antibody complexed silane surface on interdigitated electrode (IDE) sensing surface. (3-Aminopropyl)triethoxysilane linker was used to make silane-coupling with antibody and attached on the hydroxylated IDE. This electroanalytical IDE revealed the dose-dependent responses with thyroglobulin concentrations. By getting increments with the thyroglobulin concentrations, the current responses were enhanced concomitantly and the thyroglobulin detection limit was noted as 1 pM on the linear curve y = 0.1311x + 0.5386; R² = 0.9707 with the sensitivity at lower picomolar range. Moreover, the control experiments with thyroid peroxidase and nonimmune antibody cannot yield any response of current, confirming the specific detection of thyroglobulin. This research set-up is useful to determine and quantify the thyroglobulin and diagnose thyroid cancer. © 2021 International Union of Biochemistry and Molecular Biology, Inc. John Wiley and Sons Inc 2022 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101627832&doi=10.1002%2fbab.2116&partnerID=40&md5=916cac84a6bc4a04f041e514cb98e942 Wang, Y. and Sun, X. and Gopinath, S.C.B. and Saheed, M.S.M. and Wang, X. (2022) Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor. Biotechnology and Applied Biochemistry, 69 (1). pp. 376-382. http://eprints.utp.edu.my/29024/ |
| institution |
Universiti Teknologi Petronas |
| collection |
UTP Institutional Repository |
| description |
Thyroid cancer appears in endocrine glands and specific to thyroid glands has been reported widely. This work was targeted to identify and quantify thyroglobulin by using antithyroglobulin antibody complexed silane surface on interdigitated electrode (IDE) sensing surface. (3-Aminopropyl)triethoxysilane linker was used to make silane-coupling with antibody and attached on the hydroxylated IDE. This electroanalytical IDE revealed the dose-dependent responses with thyroglobulin concentrations. By getting increments with the thyroglobulin concentrations, the current responses were enhanced concomitantly and the thyroglobulin detection limit was noted as 1 pM on the linear curve y = 0.1311x + 0.5386; R² = 0.9707 with the sensitivity at lower picomolar range. Moreover, the control experiments with thyroid peroxidase and nonimmune antibody cannot yield any response of current, confirming the specific detection of thyroglobulin. This research set-up is useful to determine and quantify the thyroglobulin and diagnose thyroid cancer. © 2021 International Union of Biochemistry and Molecular Biology, Inc. |
| format |
Article |
| author |
Wang, Y. Sun, X. Gopinath, S.C.B. Saheed, M.S.M. Wang, X. |
| spellingShingle |
Wang, Y. Sun, X. Gopinath, S.C.B. Saheed, M.S.M. Wang, X. Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor |
| author_sort |
Wang, Y. |
| title |
Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor |
| title_short |
Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor |
| title_full |
Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor |
| title_fullStr |
Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor |
| title_full_unstemmed |
Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor |
| title_sort |
thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor |
| publisher |
John Wiley and Sons Inc |
| publishDate |
2022 |
| url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101627832&doi=10.1002%2fbab.2116&partnerID=40&md5=916cac84a6bc4a04f041e514cb98e942 http://eprints.utp.edu.my/29024/ |
| _version_ |
1741197184406126592 |
| score |
11.62408 |