1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen

The bovine milk allergenic protein, �β-lactoglobulin� is one of the leading causes of milk allergic reaction. In this research, a novel label-free non-faradaic capacitive aptasensor was designed to detect β-lactoglobulin using a Laser Scribed Graphene (LSG) electrode. The graphene was directly...

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Main Authors: Subramani, I.G., Perumal, V., Gopinath, S.C.B., Mohamed, N.M., Ovinis, M., Sze, L.L.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.29644 /
Published: Nature Research 2021
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117693457&doi=10.1038%2fs41598-021-00057-4&partnerID=40&md5=760e8935ae099cadaea9e718bda6aff1
http://eprints.utp.edu.my/29644/
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spelling utp-eprints.296442022-03-25T02:12:25Z 1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen Subramani, I.G. Perumal, V. Gopinath, S.C.B. Mohamed, N.M. Ovinis, M. Sze, L.L. The bovine milk allergenic protein, �β-lactoglobulin� is one of the leading causes of milk allergic reaction. In this research, a novel label-free non-faradaic capacitive aptasensor was designed to detect β-lactoglobulin using a Laser Scribed Graphene (LSG) electrode. The graphene was directly engraved into a microgapped (~ 95 µm) capacitor-electrode pattern on a flexible polyimide (PI) film via a simple one-step CO2 laser irradiation. The novel hybrid nanoflower (NF) was synthesized using 1,1�-carbonyldiimidazole (CDI) as the organic molecule and copper (Cu) as the inorganic molecule via one-pot biomineralization by tuning the reaction time and concentration. NF was fixed on the pre-modified PI film at the triangular junction of the LSG microgap specifically for bio-capturing β-lactoglobulin. The fine-tuned CDI-Cu NF revealed the flower-like structures was viewed through field emission scanning electron microscopy. Fourier-transform infrared spectroscopy showed the interactions with PI film, CDI-Cu NF, oligoaptamer and β-lactoglobulin. The non-faradaic sensing of milk allergen β-lactoglobulin corresponds to a higher loading of oligoaptamer on 3D-structured CDI-Cu NF, with a linear range detection from 1 ag/ml to 100 fg/ml and attomolar (1 ag/ml) detection limit (S/N = 3:1). This novel CDI-Cu NF/LSG microgap aptasensor has a great potential for the detection of milk allergen with high-specificity and sensitivity. © 2021, The Author(s). Nature Research 2021 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117693457&doi=10.1038%2fs41598-021-00057-4&partnerID=40&md5=760e8935ae099cadaea9e718bda6aff1 Subramani, I.G. and Perumal, V. and Gopinath, S.C.B. and Mohamed, N.M. and Ovinis, M. and Sze, L.L. (2021) 1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen. Scientific Reports, 11 (1). http://eprints.utp.edu.my/29644/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description The bovine milk allergenic protein, �β-lactoglobulin� is one of the leading causes of milk allergic reaction. In this research, a novel label-free non-faradaic capacitive aptasensor was designed to detect β-lactoglobulin using a Laser Scribed Graphene (LSG) electrode. The graphene was directly engraved into a microgapped (~ 95 µm) capacitor-electrode pattern on a flexible polyimide (PI) film via a simple one-step CO2 laser irradiation. The novel hybrid nanoflower (NF) was synthesized using 1,1�-carbonyldiimidazole (CDI) as the organic molecule and copper (Cu) as the inorganic molecule via one-pot biomineralization by tuning the reaction time and concentration. NF was fixed on the pre-modified PI film at the triangular junction of the LSG microgap specifically for bio-capturing β-lactoglobulin. The fine-tuned CDI-Cu NF revealed the flower-like structures was viewed through field emission scanning electron microscopy. Fourier-transform infrared spectroscopy showed the interactions with PI film, CDI-Cu NF, oligoaptamer and β-lactoglobulin. The non-faradaic sensing of milk allergen β-lactoglobulin corresponds to a higher loading of oligoaptamer on 3D-structured CDI-Cu NF, with a linear range detection from 1 ag/ml to 100 fg/ml and attomolar (1 ag/ml) detection limit (S/N = 3:1). This novel CDI-Cu NF/LSG microgap aptasensor has a great potential for the detection of milk allergen with high-specificity and sensitivity. © 2021, The Author(s).
format Article
author Subramani, I.G.
Perumal, V.
Gopinath, S.C.B.
Mohamed, N.M.
Ovinis, M.
Sze, L.L.
spellingShingle Subramani, I.G.
Perumal, V.
Gopinath, S.C.B.
Mohamed, N.M.
Ovinis, M.
Sze, L.L.
1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen
author_sort Subramani, I.G.
title 1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen
title_short 1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen
title_full 1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen
title_fullStr 1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen
title_full_unstemmed 1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen
title_sort 1,1'-carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen
publisher Nature Research
publishDate 2021
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117693457&doi=10.1038%2fs41598-021-00057-4&partnerID=40&md5=760e8935ae099cadaea9e718bda6aff1
http://eprints.utp.edu.my/29644/
_version_ 1741197276877946880
score 11.62408

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