Organosulfur Compounds as Soil Urease Inhibitors and Their Effect on Kinetics of Urea Hydrolysis

The application of urea fertilizer on soil surfaces causes hydrolysis reactions to occur rapidly, thus releasing ammonia gas to the atmosphere and, in turn, reducing the availability of nitrogen for plant uptake. The urease inhibitor application with urea is considered a mitigation method to reduce...

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Main Authors: Salehuddin, N.F., Mansor, N., Yahya, W.Z.N., Noor Affendi, N.M., Manogaran, M.D.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.29670 /
Published: Springer Science and Business Media Deutschland GmbH 2021
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110893571&doi=10.1007%2fs42729-021-00553-6&partnerID=40&md5=009b00e97a52c8e6a42ef46990a2f4d2
http://eprints.utp.edu.my/29670/
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spelling utp-eprints.296702022-03-25T02:29:43Z Organosulfur Compounds as Soil Urease Inhibitors and Their Effect on Kinetics of Urea Hydrolysis Salehuddin, N.F. Mansor, N. Yahya, W.Z.N. Noor Affendi, N.M. Manogaran, M.D. The application of urea fertilizer on soil surfaces causes hydrolysis reactions to occur rapidly, thus releasing ammonia gas to the atmosphere and, in turn, reducing the availability of nitrogen for plant uptake. The urease inhibitor application with urea is considered a mitigation method to reduce the loss of nitrogen. Chemical-based urease inhibitors effectively reduce nitrogen loss, but they also demonstrate adverse side effects on plants. Thus, there is a need to discover potential candidates for urease inhibitors from natural sources. This study evaluates the potential of organosulfur compounds from garlic, namely, allicin, diallyl sulfide (DAS), and diallyl disulfide (DADS), as inhibitors to urea hydrolysis. The effect of allicin, DAS, and DADS on kinetic parameters of soil urease and urea hydrolysis rate was evaluated using the Lineweaver�Burk plot and incubation study. All tested organosulfur compounds exhibited mixed-type inhibition with a high Michaelis constant (Km) and low maximum velocity of reaction (Vmax), compared with control (urea). The calculated inhibitory (dissociation) constant (Ki) for allicin, DAS, and DADS were 0.53, 0.92, and 0.40 mM, respectively. This indicates that DADS is the most potent soil urease inhibitor, followed by allicin and DAS. After DADS treatment, about 46 of the urea-nitrogen (N) content remained after 21 days. Meanwhile, allicin and DAS treatments retained urea-N in the soil for 7 and 11 days, respectively. The results demonstrate that DADS is more effective in delaying enzymatic urea hydrolysis compared with allicin and DAS and showed a high potential as a bio-based urease inhibitor in agriculture. © 2021, Sociedad Chilena de la Ciencia del Suelo. Springer Science and Business Media Deutschland GmbH 2021 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110893571&doi=10.1007%2fs42729-021-00553-6&partnerID=40&md5=009b00e97a52c8e6a42ef46990a2f4d2 Salehuddin, N.F. and Mansor, N. and Yahya, W.Z.N. and Noor Affendi, N.M. and Manogaran, M.D. (2021) Organosulfur Compounds as Soil Urease Inhibitors and Their Effect on Kinetics of Urea Hydrolysis. Journal of Soil Science and Plant Nutrition, 21 (4). pp. 2652-2659. http://eprints.utp.edu.my/29670/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description The application of urea fertilizer on soil surfaces causes hydrolysis reactions to occur rapidly, thus releasing ammonia gas to the atmosphere and, in turn, reducing the availability of nitrogen for plant uptake. The urease inhibitor application with urea is considered a mitigation method to reduce the loss of nitrogen. Chemical-based urease inhibitors effectively reduce nitrogen loss, but they also demonstrate adverse side effects on plants. Thus, there is a need to discover potential candidates for urease inhibitors from natural sources. This study evaluates the potential of organosulfur compounds from garlic, namely, allicin, diallyl sulfide (DAS), and diallyl disulfide (DADS), as inhibitors to urea hydrolysis. The effect of allicin, DAS, and DADS on kinetic parameters of soil urease and urea hydrolysis rate was evaluated using the Lineweaver�Burk plot and incubation study. All tested organosulfur compounds exhibited mixed-type inhibition with a high Michaelis constant (Km) and low maximum velocity of reaction (Vmax), compared with control (urea). The calculated inhibitory (dissociation) constant (Ki) for allicin, DAS, and DADS were 0.53, 0.92, and 0.40 mM, respectively. This indicates that DADS is the most potent soil urease inhibitor, followed by allicin and DAS. After DADS treatment, about 46 of the urea-nitrogen (N) content remained after 21 days. Meanwhile, allicin and DAS treatments retained urea-N in the soil for 7 and 11 days, respectively. The results demonstrate that DADS is more effective in delaying enzymatic urea hydrolysis compared with allicin and DAS and showed a high potential as a bio-based urease inhibitor in agriculture. © 2021, Sociedad Chilena de la Ciencia del Suelo.
format Article
author Salehuddin, N.F.
Mansor, N.
Yahya, W.Z.N.
Noor Affendi, N.M.
Manogaran, M.D.
spellingShingle Salehuddin, N.F.
Mansor, N.
Yahya, W.Z.N.
Noor Affendi, N.M.
Manogaran, M.D.
Organosulfur Compounds as Soil Urease Inhibitors and Their Effect on Kinetics of Urea Hydrolysis
author_sort Salehuddin, N.F.
title Organosulfur Compounds as Soil Urease Inhibitors and Their Effect on Kinetics of Urea Hydrolysis
title_short Organosulfur Compounds as Soil Urease Inhibitors and Their Effect on Kinetics of Urea Hydrolysis
title_full Organosulfur Compounds as Soil Urease Inhibitors and Their Effect on Kinetics of Urea Hydrolysis
title_fullStr Organosulfur Compounds as Soil Urease Inhibitors and Their Effect on Kinetics of Urea Hydrolysis
title_full_unstemmed Organosulfur Compounds as Soil Urease Inhibitors and Their Effect on Kinetics of Urea Hydrolysis
title_sort organosulfur compounds as soil urease inhibitors and their effect on kinetics of urea hydrolysis
publisher Springer Science and Business Media Deutschland GmbH
publishDate 2021
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85110893571&doi=10.1007%2fs42729-021-00553-6&partnerID=40&md5=009b00e97a52c8e6a42ef46990a2f4d2
http://eprints.utp.edu.my/29670/
_version_ 1741197280992559104
score 11.62408

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