3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination

Recently, non-faradaic electrochemical impedimetric (nfEIS) has been identified as a dynamic and robust method for bio-determining. However, the success of a nfEIS approach largely depends on its sensing layer architecture. In this study, the use of a Glutaraldehyde (GA) crosslinked copper (Cu) �...

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Main Authors: Subramani, I.G., Perumal, V., Gopinath, S.C.B., Mohamed, N.M., Joshi, N., Ovinis, M., Sze, L.L.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.29820 /
Published: Taiwan Institute of Chemical Engineers 2020
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096882369&doi=10.1016%2fj.jtice.2020.11.006&partnerID=40&md5=dc9ec9e69b1cf9f8e68c3cc50db7502b
http://eprints.utp.edu.my/29820/
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spelling utp-eprints.298202022-03-25T02:56:55Z 3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination Subramani, I.G. Perumal, V. Gopinath, S.C.B. Mohamed, N.M. Joshi, N. Ovinis, M. Sze, L.L. Recently, non-faradaic electrochemical impedimetric (nfEIS) has been identified as a dynamic and robust method for bio-determining. However, the success of a nfEIS approach largely depends on its sensing layer architecture. In this study, the use of a Glutaraldehyde (GA) crosslinked copper (Cu) � bovine serum albumin (BSA) hybrid nanoflower with three-dimensional nanoporous architecture for the sensing layer was investigated. The nanoflower morphology changes were observed under FESEM, revealing loosely interlaced nanoflower into a tightly interlaced, highly porous structure upon GA crosslinking. This nanoflower was hybridized to immobilize aminated-DNA probe on the transducer surface and detect the target TB DNA in their natural redox-free environment. FTIR and XPS characterization showed distinct peaks at 950�1100 cm�1 (P-O, P=O bonds from nanoflower and DNA backbone) and 286.48 eV (interaction between BSA and aminated DNA), respectively, validating the successful DNA probe immobilization on the nanoflower surface. Furthermore, impedimetric sensing in a redox-free environment showed that the developed TB biosensor present has a detection limit (LOD) of 60 pM with a (linear) range from 1 pM to 1 µM with good reproducibility. This redox-free non-faradaic EIS offers excellent biosensing potential and may be extended for diagnosing other biomarkers in clinical practice. © 2020 Taiwan Institute of Chemical Engineers Taiwan Institute of Chemical Engineers 2020 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096882369&doi=10.1016%2fj.jtice.2020.11.006&partnerID=40&md5=dc9ec9e69b1cf9f8e68c3cc50db7502b Subramani, I.G. and Perumal, V. and Gopinath, S.C.B. and Mohamed, N.M. and Joshi, N. and Ovinis, M. and Sze, L.L. (2020) 3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination. Journal of the Taiwan Institute of Chemical Engineers, 116 . pp. 26-35. http://eprints.utp.edu.my/29820/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description Recently, non-faradaic electrochemical impedimetric (nfEIS) has been identified as a dynamic and robust method for bio-determining. However, the success of a nfEIS approach largely depends on its sensing layer architecture. In this study, the use of a Glutaraldehyde (GA) crosslinked copper (Cu) � bovine serum albumin (BSA) hybrid nanoflower with three-dimensional nanoporous architecture for the sensing layer was investigated. The nanoflower morphology changes were observed under FESEM, revealing loosely interlaced nanoflower into a tightly interlaced, highly porous structure upon GA crosslinking. This nanoflower was hybridized to immobilize aminated-DNA probe on the transducer surface and detect the target TB DNA in their natural redox-free environment. FTIR and XPS characterization showed distinct peaks at 950�1100 cm�1 (P-O, P=O bonds from nanoflower and DNA backbone) and 286.48 eV (interaction between BSA and aminated DNA), respectively, validating the successful DNA probe immobilization on the nanoflower surface. Furthermore, impedimetric sensing in a redox-free environment showed that the developed TB biosensor present has a detection limit (LOD) of 60 pM with a (linear) range from 1 pM to 1 µM with good reproducibility. This redox-free non-faradaic EIS offers excellent biosensing potential and may be extended for diagnosing other biomarkers in clinical practice. © 2020 Taiwan Institute of Chemical Engineers
format Article
author Subramani, I.G.
Perumal, V.
Gopinath, S.C.B.
Mohamed, N.M.
Joshi, N.
Ovinis, M.
Sze, L.L.
spellingShingle Subramani, I.G.
Perumal, V.
Gopinath, S.C.B.
Mohamed, N.M.
Joshi, N.
Ovinis, M.
Sze, L.L.
3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination
author_sort Subramani, I.G.
title 3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination
title_short 3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination
title_full 3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination
title_fullStr 3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination
title_full_unstemmed 3D nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination
title_sort 3d nanoporous hybrid nanoflower for enhanced non-faradaic redox-free electrochemical impedimetric biodetermination
publisher Taiwan Institute of Chemical Engineers
publishDate 2020
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096882369&doi=10.1016%2fj.jtice.2020.11.006&partnerID=40&md5=dc9ec9e69b1cf9f8e68c3cc50db7502b
http://eprints.utp.edu.my/29820/
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score 11.62408

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