Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection

The analysis of regulated contaminants in fruit drinks often requires suitable validated and rapid analytical methods for cost-effective food control, and is of considerable interest among the fruit beverage industry. This study demonstrated a rapid and sensitive high-performance liquid chromatograp...

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Main Authors: Hassan, N.H., Al Othman, H.I.A., Malek, N.R.A., Zulkurnain, M., Saad, B., Wong, Y.F.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.29850 /
Published: MDPI 2020
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103380995&doi=10.3390%2ffoods9111633&partnerID=40&md5=453cd8b59f3055a7ecf007a76c933b46
http://eprints.utp.edu.my/29850/
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spelling utp-eprints.298502022-03-25T02:57:28Z Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection Hassan, N.H. Al Othman, H.I.A. Malek, N.R.A. Zulkurnain, M. Saad, B. Wong, Y.F. The analysis of regulated contaminants in fruit drinks often requires suitable validated and rapid analytical methods for cost-effective food control, and is of considerable interest among the fruit beverage industry. This study demonstrated a rapid and sensitive high-performance liquid chromatography approach for the simultaneous determination of ochratoxin A (OTA), patulin (PAT), 5-hydroxymethylfurfural (HMF), and bisphenol A (BPA) in various fruit drinks. The separations were achieved using a C18 core-shell column with both photo-diode array and fluorimetric detections connected in series. A gradient system consisting of methanol and 0.1 formic acid at a flow rate of 1.2 mL min�1, thermostated at 35� C, provided fast elution with run time <9 min. Sample pretreatment was optimised to enable extraction of all analytes from fruit drink matrices. The optimised method was validated. Correlation coefficients of R > 0.99 were achieved with detection limits of 0.5 ng mL�1 (OTA), 1.1 ng mL�1 (PAT), 7.9 ng mL�1 (HMF), and 1.0 ng mL�1 (BPA). Recoveries ranged from 82 to 99. Good relative standard deviations for intraday retention times (�3.54) and peak area (�3.5) were achieved. The developed multi-contaminants analysis method was successfully applied to determine OTA, PAT, HMF, and BPA in various fruit drinks. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. MDPI 2020 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103380995&doi=10.3390%2ffoods9111633&partnerID=40&md5=453cd8b59f3055a7ecf007a76c933b46 Hassan, N.H. and Al Othman, H.I.A. and Malek, N.R.A. and Zulkurnain, M. and Saad, B. and Wong, Y.F. (2020) Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection. Foods, 9 (11). http://eprints.utp.edu.my/29850/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description The analysis of regulated contaminants in fruit drinks often requires suitable validated and rapid analytical methods for cost-effective food control, and is of considerable interest among the fruit beverage industry. This study demonstrated a rapid and sensitive high-performance liquid chromatography approach for the simultaneous determination of ochratoxin A (OTA), patulin (PAT), 5-hydroxymethylfurfural (HMF), and bisphenol A (BPA) in various fruit drinks. The separations were achieved using a C18 core-shell column with both photo-diode array and fluorimetric detections connected in series. A gradient system consisting of methanol and 0.1 formic acid at a flow rate of 1.2 mL min�1, thermostated at 35� C, provided fast elution with run time <9 min. Sample pretreatment was optimised to enable extraction of all analytes from fruit drink matrices. The optimised method was validated. Correlation coefficients of R > 0.99 were achieved with detection limits of 0.5 ng mL�1 (OTA), 1.1 ng mL�1 (PAT), 7.9 ng mL�1 (HMF), and 1.0 ng mL�1 (BPA). Recoveries ranged from 82 to 99. Good relative standard deviations for intraday retention times (�3.54) and peak area (�3.5) were achieved. The developed multi-contaminants analysis method was successfully applied to determine OTA, PAT, HMF, and BPA in various fruit drinks. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
format Article
author Hassan, N.H.
Al Othman, H.I.A.
Malek, N.R.A.
Zulkurnain, M.
Saad, B.
Wong, Y.F.
spellingShingle Hassan, N.H.
Al Othman, H.I.A.
Malek, N.R.A.
Zulkurnain, M.
Saad, B.
Wong, Y.F.
Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection
author_sort Hassan, N.H.
title Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection
title_short Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection
title_full Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection
title_fullStr Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection
title_full_unstemmed Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection
title_sort simultaneous quantitative assessment of ochratoxin a, patulin, 5-hydroxymethylfurfural, and bisphenol a in fruit drinks using hplc with diode array-fluorimetric detection
publisher MDPI
publishDate 2020
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103380995&doi=10.3390%2ffoods9111633&partnerID=40&md5=453cd8b59f3055a7ecf007a76c933b46
http://eprints.utp.edu.my/29850/
_version_ 1741197309189816320
score 11.62408

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