Evaluation and detoxification of aflatoxins in ground and tree nuts using food grade organic acids

The contamination of foodstuffs especially nuts with aflatoxins (AFs) affected by some of the fungal genera species is a major threat to the economy, safe food supply and serious health concerns to any country in recent days. Recently different techniques including heat, ozone and microbes are used...

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Main Authors: Jubeen, F., Sher, F., Hazafa, A., Zafar, F., Ameen, M., Rasheed, T.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.29947 /
Published: Elsevier Ltd 2020
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091627260&doi=10.1016%2fj.bcab.2020.101749&partnerID=40&md5=2fb427de4f0fc4bcffee312b68e4b2ef
http://eprints.utp.edu.my/29947/
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spelling utp-eprints.299472022-03-25T03:14:57Z Evaluation and detoxification of aflatoxins in ground and tree nuts using food grade organic acids Jubeen, F. Sher, F. Hazafa, A. Zafar, F. Ameen, M. Rasheed, T. The contamination of foodstuffs especially nuts with aflatoxins (AFs) affected by some of the fungal genera species is a major threat to the economy, safe food supply and serious health concerns to any country in recent days. Recently different techniques including heat, ozone and microbes are used for the decontamination of aflatoxin but all these are limited to achieve the desirable results. The present study objectives to decontaminate AFs in nuts by using three food-grade organic acids. The aqueous solutions of three food-grade organic acids namely citric, lactic and propionic acid are used at five different concentrations (1, 3, 5, 7 and 9) to detoxify aflatoxin B1 (AFB1) and total aflatoxins (TAFs) that includes;AFB1, AFB2, AFG1 and AFG2 in selected nuts including almond, peanut, pistachio and walnut at two different moisture levels (10 ± 3 and 16 ± 3). The high-performance liquid chromatography (HPLC) coupled with fluorescence detection method was applied for the qualitative and quantitative determination of AFs. The results showed that the decontamination of AFB1 and TAFs significantly increased in infected nuts by increasing the concentration of acids. The experimental results after 15 min treatment of walnut (10 ± 3 and 16 ± 3 moisture level), pistachio (10 ± 3 moisture content) and peanuts (10 ± 3 moisture content) with citric, lactic and propionic acids at 9 concentration significantly reduced AFs about 99, 99.90 and 96.07 respectively. Furthermore, treatment with citric and lactic acids resulted in the conversion of AFB1 into less toxic products identified as AFD1 via hydrolysis of the lactone ring. Furthermore, citric acid was found as the most efficient acid in degrading the TAFs among all three organic acids. The present study showed better AFs detoxification results than conventional methods. Hence, it is concluded that citric, lactic, and propionic acids can be applied as a useful and safe decontamination method for AFB1 and TAFs in aflatoxin-affected nuts. © 2020 Elsevier Ltd Elsevier Ltd 2020 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091627260&doi=10.1016%2fj.bcab.2020.101749&partnerID=40&md5=2fb427de4f0fc4bcffee312b68e4b2ef Jubeen, F. and Sher, F. and Hazafa, A. and Zafar, F. and Ameen, M. and Rasheed, T. (2020) Evaluation and detoxification of aflatoxins in ground and tree nuts using food grade organic acids. Biocatalysis and Agricultural Biotechnology, 29 . http://eprints.utp.edu.my/29947/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description The contamination of foodstuffs especially nuts with aflatoxins (AFs) affected by some of the fungal genera species is a major threat to the economy, safe food supply and serious health concerns to any country in recent days. Recently different techniques including heat, ozone and microbes are used for the decontamination of aflatoxin but all these are limited to achieve the desirable results. The present study objectives to decontaminate AFs in nuts by using three food-grade organic acids. The aqueous solutions of three food-grade organic acids namely citric, lactic and propionic acid are used at five different concentrations (1, 3, 5, 7 and 9) to detoxify aflatoxin B1 (AFB1) and total aflatoxins (TAFs) that includes;AFB1, AFB2, AFG1 and AFG2 in selected nuts including almond, peanut, pistachio and walnut at two different moisture levels (10 ± 3 and 16 ± 3). The high-performance liquid chromatography (HPLC) coupled with fluorescence detection method was applied for the qualitative and quantitative determination of AFs. The results showed that the decontamination of AFB1 and TAFs significantly increased in infected nuts by increasing the concentration of acids. The experimental results after 15 min treatment of walnut (10 ± 3 and 16 ± 3 moisture level), pistachio (10 ± 3 moisture content) and peanuts (10 ± 3 moisture content) with citric, lactic and propionic acids at 9 concentration significantly reduced AFs about 99, 99.90 and 96.07 respectively. Furthermore, treatment with citric and lactic acids resulted in the conversion of AFB1 into less toxic products identified as AFD1 via hydrolysis of the lactone ring. Furthermore, citric acid was found as the most efficient acid in degrading the TAFs among all three organic acids. The present study showed better AFs detoxification results than conventional methods. Hence, it is concluded that citric, lactic, and propionic acids can be applied as a useful and safe decontamination method for AFB1 and TAFs in aflatoxin-affected nuts. © 2020 Elsevier Ltd
format Article
author Jubeen, F.
Sher, F.
Hazafa, A.
Zafar, F.
Ameen, M.
Rasheed, T.
spellingShingle Jubeen, F.
Sher, F.
Hazafa, A.
Zafar, F.
Ameen, M.
Rasheed, T.
Evaluation and detoxification of aflatoxins in ground and tree nuts using food grade organic acids
author_sort Jubeen, F.
title Evaluation and detoxification of aflatoxins in ground and tree nuts using food grade organic acids
title_short Evaluation and detoxification of aflatoxins in ground and tree nuts using food grade organic acids
title_full Evaluation and detoxification of aflatoxins in ground and tree nuts using food grade organic acids
title_fullStr Evaluation and detoxification of aflatoxins in ground and tree nuts using food grade organic acids
title_full_unstemmed Evaluation and detoxification of aflatoxins in ground and tree nuts using food grade organic acids
title_sort evaluation and detoxification of aflatoxins in ground and tree nuts using food grade organic acids
publisher Elsevier Ltd
publishDate 2020
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85091627260&doi=10.1016%2fj.bcab.2020.101749&partnerID=40&md5=2fb427de4f0fc4bcffee312b68e4b2ef
http://eprints.utp.edu.my/29947/
_version_ 1741197325045334016
score 11.62408

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