Preliminary in vitro evaluation of chitosan�graphene oxide scaffolds on osteoblastic adhesion, proliferation, and early differentiation

An ideal scaffold should be biocompatible, having appropriate microstructure, excellent mechanical strength yet degrades. Chitosan exhibits most of these exceptional properties, but it is always associated with sub-optimal cytocompatibility. This study aimed to incorporate graphene oxide at wt of 0...

Full description

Main Authors: Wong, S.H.M., Lim, S.S., Tiong, T.J., Show, P.L., Zaid, H.F.M., Loh, H.-S.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.30106 /
Published: MDPI AG 2020
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088304192&doi=10.3390%2fijms21155202&partnerID=40&md5=05773e178cca9c811be4904222404b91
http://eprints.utp.edu.my/30106/
Tags: Add Tag
No Tags, Be the first to tag this record!
id utp-eprints.30106
recordtype eprints
spelling utp-eprints.301062022-03-25T06:34:39Z Preliminary in vitro evaluation of chitosan�graphene oxide scaffolds on osteoblastic adhesion, proliferation, and early differentiation Wong, S.H.M. Lim, S.S. Tiong, T.J. Show, P.L. Zaid, H.F.M. Loh, H.-S. An ideal scaffold should be biocompatible, having appropriate microstructure, excellent mechanical strength yet degrades. Chitosan exhibits most of these exceptional properties, but it is always associated with sub-optimal cytocompatibility. This study aimed to incorporate graphene oxide at wt of 0, 2, 4, and 6 into chitosan matrix via direct blending of chitosan solution and graphene oxide, freezing, and freeze drying. Cell fixation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, alkaline phosphatase colorimetric assays were conducted to assess cell adhesion, proliferation, and early differentiation of MG63 on chitosan�graphene oxide scaffolds respectively. The presence of alkaline phosphatase, an early osteoblast differentiation marker, was further detected in chitosan�graphene oxide scaffolds using western blot. These results strongly supported that chitosan scaffolds loaded with graphene oxide at 2 wt mediated cell adhesion, proliferation, and early differentiation due to the presence of oxygen-containing functional groups of graphene oxide. Therefore, chitosan scaffolds loaded with graphene oxide at 2 wt showed the potential to be developed into functional bone scaffolds. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. MDPI AG 2020 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088304192&doi=10.3390%2fijms21155202&partnerID=40&md5=05773e178cca9c811be4904222404b91 Wong, S.H.M. and Lim, S.S. and Tiong, T.J. and Show, P.L. and Zaid, H.F.M. and Loh, H.-S. (2020) Preliminary in vitro evaluation of chitosan�graphene oxide scaffolds on osteoblastic adhesion, proliferation, and early differentiation. International Journal of Molecular Sciences, 21 (15). pp. 1-12. http://eprints.utp.edu.my/30106/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description An ideal scaffold should be biocompatible, having appropriate microstructure, excellent mechanical strength yet degrades. Chitosan exhibits most of these exceptional properties, but it is always associated with sub-optimal cytocompatibility. This study aimed to incorporate graphene oxide at wt of 0, 2, 4, and 6 into chitosan matrix via direct blending of chitosan solution and graphene oxide, freezing, and freeze drying. Cell fixation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, alkaline phosphatase colorimetric assays were conducted to assess cell adhesion, proliferation, and early differentiation of MG63 on chitosan�graphene oxide scaffolds respectively. The presence of alkaline phosphatase, an early osteoblast differentiation marker, was further detected in chitosan�graphene oxide scaffolds using western blot. These results strongly supported that chitosan scaffolds loaded with graphene oxide at 2 wt mediated cell adhesion, proliferation, and early differentiation due to the presence of oxygen-containing functional groups of graphene oxide. Therefore, chitosan scaffolds loaded with graphene oxide at 2 wt showed the potential to be developed into functional bone scaffolds. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
format Article
author Wong, S.H.M.
Lim, S.S.
Tiong, T.J.
Show, P.L.
Zaid, H.F.M.
Loh, H.-S.
spellingShingle Wong, S.H.M.
Lim, S.S.
Tiong, T.J.
Show, P.L.
Zaid, H.F.M.
Loh, H.-S.
Preliminary in vitro evaluation of chitosan�graphene oxide scaffolds on osteoblastic adhesion, proliferation, and early differentiation
author_sort Wong, S.H.M.
title Preliminary in vitro evaluation of chitosan�graphene oxide scaffolds on osteoblastic adhesion, proliferation, and early differentiation
title_short Preliminary in vitro evaluation of chitosan�graphene oxide scaffolds on osteoblastic adhesion, proliferation, and early differentiation
title_full Preliminary in vitro evaluation of chitosan�graphene oxide scaffolds on osteoblastic adhesion, proliferation, and early differentiation
title_fullStr Preliminary in vitro evaluation of chitosan�graphene oxide scaffolds on osteoblastic adhesion, proliferation, and early differentiation
title_full_unstemmed Preliminary in vitro evaluation of chitosan�graphene oxide scaffolds on osteoblastic adhesion, proliferation, and early differentiation
title_sort preliminary in vitro evaluation of chitosan�graphene oxide scaffolds on osteoblastic adhesion, proliferation, and early differentiation
publisher MDPI AG
publishDate 2020
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85088304192&doi=10.3390%2fijms21155202&partnerID=40&md5=05773e178cca9c811be4904222404b91
http://eprints.utp.edu.my/30106/
_version_ 1741197350827720704
score 11.62408

Similar Items