Microbial production of poly (3-hydroxybutyrate) (PHB) from rubber seed oil using Cupriavidus necator H16

Rubber seed oil is inedible plant oil that is a potential replacement for the traditional organic building blocks used in PHB biosynthesis. Herein we describe the biosynthesis of PHB by Cupriavidus necator H16 grown in media with rubber seed oil and urea serving as carbon and nitrogen sources, respe...

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Main Authors: Reddy Prasad, D.M., Pendyala, R., Senthilkumar, R., Azri, M.H.B.
Format: Conference or Workshop Item
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.30132 /
Published: Institute of Physics Publishing 2019
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078804140&doi=10.1088%2f1755-1315%2f398%2f1%2f012008&partnerID=40&md5=402f2b248bdd5851526f49aa49202b11
http://eprints.utp.edu.my/30132/
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spelling utp-eprints.301322022-03-25T06:35:07Z Microbial production of poly (3-hydroxybutyrate) (PHB) from rubber seed oil using Cupriavidus necator H16 Reddy Prasad, D.M. Pendyala, R. Senthilkumar, R. Azri, M.H.B. Rubber seed oil is inedible plant oil that is a potential replacement for the traditional organic building blocks used in PHB biosynthesis. Herein we describe the biosynthesis of PHB by Cupriavidus necator H16 grown in media with rubber seed oil and urea serving as carbon and nitrogen sources, respectively. The method was optimized over a temperature range of 30-50 °C for batch culture studies. A maximized PHB concentration of 15.4 g/L, and dry well weight of 19.2 g/L was obtained using 20 g/L of rubber seed oil at 30°C. Experimental results were used to generate a logistic curve, and the Luedeking-Piret model was implemented to predict the specific growth rate of C. necator at 0.1898 1/h. The estimated rate constants were in good agreement with experimental values. This study demonstrated that rubber seed oil is a viable alternative to conventional carbon sources for the production of PHB using C.necator H16. © 2019 Published under licence by IOP Publishing Ltd. Institute of Physics Publishing 2019 Conference or Workshop Item NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078804140&doi=10.1088%2f1755-1315%2f398%2f1%2f012008&partnerID=40&md5=402f2b248bdd5851526f49aa49202b11 Reddy Prasad, D.M. and Pendyala, R. and Senthilkumar, R. and Azri, M.H.B. (2019) Microbial production of poly (3-hydroxybutyrate) (PHB) from rubber seed oil using Cupriavidus necator H16. In: UNSPECIFIED. http://eprints.utp.edu.my/30132/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description Rubber seed oil is inedible plant oil that is a potential replacement for the traditional organic building blocks used in PHB biosynthesis. Herein we describe the biosynthesis of PHB by Cupriavidus necator H16 grown in media with rubber seed oil and urea serving as carbon and nitrogen sources, respectively. The method was optimized over a temperature range of 30-50 °C for batch culture studies. A maximized PHB concentration of 15.4 g/L, and dry well weight of 19.2 g/L was obtained using 20 g/L of rubber seed oil at 30°C. Experimental results were used to generate a logistic curve, and the Luedeking-Piret model was implemented to predict the specific growth rate of C. necator at 0.1898 1/h. The estimated rate constants were in good agreement with experimental values. This study demonstrated that rubber seed oil is a viable alternative to conventional carbon sources for the production of PHB using C.necator H16. © 2019 Published under licence by IOP Publishing Ltd.
format Conference or Workshop Item
author Reddy Prasad, D.M.
Pendyala, R.
Senthilkumar, R.
Azri, M.H.B.
spellingShingle Reddy Prasad, D.M.
Pendyala, R.
Senthilkumar, R.
Azri, M.H.B.
Microbial production of poly (3-hydroxybutyrate) (PHB) from rubber seed oil using Cupriavidus necator H16
author_sort Reddy Prasad, D.M.
title Microbial production of poly (3-hydroxybutyrate) (PHB) from rubber seed oil using Cupriavidus necator H16
title_short Microbial production of poly (3-hydroxybutyrate) (PHB) from rubber seed oil using Cupriavidus necator H16
title_full Microbial production of poly (3-hydroxybutyrate) (PHB) from rubber seed oil using Cupriavidus necator H16
title_fullStr Microbial production of poly (3-hydroxybutyrate) (PHB) from rubber seed oil using Cupriavidus necator H16
title_full_unstemmed Microbial production of poly (3-hydroxybutyrate) (PHB) from rubber seed oil using Cupriavidus necator H16
title_sort microbial production of poly (3-hydroxybutyrate) (phb) from rubber seed oil using cupriavidus necator h16
publisher Institute of Physics Publishing
publishDate 2019
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078804140&doi=10.1088%2f1755-1315%2f398%2f1%2f012008&partnerID=40&md5=402f2b248bdd5851526f49aa49202b11
http://eprints.utp.edu.my/30132/
_version_ 1741197355029364736
score 11.62408

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