Biodegradation of H acid by Bacillus subtilis and RP-HPLC method development for percent degradation estimation

The industrial synthesis of pigments and dyes generates hazardous chemicals. These chemicals are released into water, soil and environment and regarded as a major source of environmental pollution. H acid is also an important dye intermediate. In this study, the focus was on the bacterial degradatio...

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Main Authors: Khan, R.R.M., Adnan, A., Pervaiz, M., Raza, M.A., Sagir, M., Naz, M.Y.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.30963 /
Published: Maik Nauka Publishing / Springer SBM 2016
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978501331&doi=10.1134%2fS1990793116030234&partnerID=40&md5=87b6da923a93d866730910d903f00d95
http://eprints.utp.edu.my/30963/
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spelling utp-eprints.309632022-03-25T07:52:08Z Biodegradation of H acid by Bacillus subtilis and RP-HPLC method development for percent degradation estimation Khan, R.R.M. Adnan, A. Pervaiz, M. Raza, M.A. Sagir, M. Naz, M.Y. The industrial synthesis of pigments and dyes generates hazardous chemicals. These chemicals are released into water, soil and environment and regarded as a major source of environmental pollution. H acid is also an important dye intermediate. In this study, the focus was on the bacterial degradation of H acid (1-amino-8-naphthol-3,6-disulfonic acid) of different concentrations and its degradation estimation method. The biodegradation was identified by the help of IR, UV-Visible spectrophotometer and high performance liquid chromatography (HPLC). A method was developed for the estimation of percent biodegradation of H acid with the help of HPLC. The developed method was applicable on standard as well as biodegraded products and on other compounds with the sulfonic acid group. It was found that H acid of concentration 300 ppm and below can be completely degraded with Bacillus subtilis. However, over 300 ppm, the bacterial degradation did not work efficiently. © 2016, Pleiades Publishing, Ltd. Maik Nauka Publishing / Springer SBM 2016 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978501331&doi=10.1134%2fS1990793116030234&partnerID=40&md5=87b6da923a93d866730910d903f00d95 Khan, R.R.M. and Adnan, A. and Pervaiz, M. and Raza, M.A. and Sagir, M. and Naz, M.Y. (2016) Biodegradation of H acid by Bacillus subtilis and RP-HPLC method development for percent degradation estimation. Russian Journal of Physical Chemistry B, 10 (3). pp. 517-523. http://eprints.utp.edu.my/30963/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description The industrial synthesis of pigments and dyes generates hazardous chemicals. These chemicals are released into water, soil and environment and regarded as a major source of environmental pollution. H acid is also an important dye intermediate. In this study, the focus was on the bacterial degradation of H acid (1-amino-8-naphthol-3,6-disulfonic acid) of different concentrations and its degradation estimation method. The biodegradation was identified by the help of IR, UV-Visible spectrophotometer and high performance liquid chromatography (HPLC). A method was developed for the estimation of percent biodegradation of H acid with the help of HPLC. The developed method was applicable on standard as well as biodegraded products and on other compounds with the sulfonic acid group. It was found that H acid of concentration 300 ppm and below can be completely degraded with Bacillus subtilis. However, over 300 ppm, the bacterial degradation did not work efficiently. © 2016, Pleiades Publishing, Ltd.
format Article
author Khan, R.R.M.
Adnan, A.
Pervaiz, M.
Raza, M.A.
Sagir, M.
Naz, M.Y.
spellingShingle Khan, R.R.M.
Adnan, A.
Pervaiz, M.
Raza, M.A.
Sagir, M.
Naz, M.Y.
Biodegradation of H acid by Bacillus subtilis and RP-HPLC method development for percent degradation estimation
author_sort Khan, R.R.M.
title Biodegradation of H acid by Bacillus subtilis and RP-HPLC method development for percent degradation estimation
title_short Biodegradation of H acid by Bacillus subtilis and RP-HPLC method development for percent degradation estimation
title_full Biodegradation of H acid by Bacillus subtilis and RP-HPLC method development for percent degradation estimation
title_fullStr Biodegradation of H acid by Bacillus subtilis and RP-HPLC method development for percent degradation estimation
title_full_unstemmed Biodegradation of H acid by Bacillus subtilis and RP-HPLC method development for percent degradation estimation
title_sort biodegradation of h acid by bacillus subtilis and rp-hplc method development for percent degradation estimation
publisher Maik Nauka Publishing / Springer SBM
publishDate 2016
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978501331&doi=10.1134%2fS1990793116030234&partnerID=40&md5=87b6da923a93d866730910d903f00d95
http://eprints.utp.edu.my/30963/
_version_ 1741197496499044352
score 11.62408

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