Production of bioethanol from empty fruit bunches cellulosic biomass and Avicel PH-101 cellulose

Cellulosic ethanol production was carried out using empty fruit bunches (EFB) via simultaneous saccharification and fermentation (SSF) method. The EFB was pretreated with alkaline treatment using 0.2Â M of sodium hydroxide (NaOH) solution followed by mild acid hydrolysis using 3Â of sulfuric acid (...

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Main Authors: Karim, R.A., Hussain, A.S., Mohd Zain, A.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.31060 /
Published: Springer Verlag 2014
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84978024448&doi=10.1007%2fs13399-014-0117-7&partnerID=40&md5=78c43585140280448bc45da98c50dde8
http://eprints.utp.edu.my/31060/
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Summary: Cellulosic ethanol production was carried out using empty fruit bunches (EFB) via simultaneous saccharification and fermentation (SSF) method. The EFB was pretreated with alkaline treatment using 0.2 M of sodium hydroxide (NaOH) solution followed by mild acid hydrolysis using 3 of sulfuric acid (H2SO4), and enzymatic saccharification using derived cellulose, Trichoderma reesei prior to fermentation using Saccharomyces cerevisiae. Acid hydrolysis-pretreated samples shows the best substrates to be used in fermentation since it can produce the highest amount of glucose and highest percentage of saccharification with 5.3 mg/mL and 48 , respectively. The EFB hydrolyzate obtained was subjected to fermentation under anaerobic condition. It was found that the highest ethanol yield was 0.42 mg/mL from acid hydrolyze sample. Optimization of SSF was conducted on filter paper unit (FPU), pH and mass loading effect for bioethanol production. Highest ethanol productions from cellulose (Avicel PH-101) are 3.1, 3.7, and 4.6 mg/mL using FPU 217, pH 4, and a 5.0-g cellulose loading accordingly. © 2014, Springer-Verlag Berlin Heidelberg.