Mxene surface on multiple junction triangles for determining osteosarcoma cancer biomarker by dielectrode microgap sensor

Background: In recent years, nanomaterials have justified their dissemination for biosensor application towards the sensitive and selective detections of clinical biomarkers at the lower levels. MXene is a two-dimensional layered transition metal, attractive for biosensing due to its chemical, physi...

Full description

Main Authors: Zhou, D., Gopinath, S.C.B., Mohamed Saheed, M.S., Siva Sangu, S., Lakshmipriya, T.
Format: Article
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.32408 /
Published: Dove Medical Press Ltd 2020
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85098218768&doi=10.2147%2fIJN.S284752&partnerID=40&md5=9c8e2699d1af1ab60f7f08f20edebada
http://eprints.utp.edu.my/32408/
Tags: Add Tag
No Tags, Be the first to tag this record!
id utp-eprints.32408
recordtype eprints
spelling utp-eprints.324082022-03-29T01:55:12Z Mxene surface on multiple junction triangles for determining osteosarcoma cancer biomarker by dielectrode microgap sensor Zhou, D. Gopinath, S.C.B. Mohamed Saheed, M.S. Siva Sangu, S. Lakshmipriya, T. Background: In recent years, nanomaterials have justified their dissemination for biosensor application towards the sensitive and selective detections of clinical biomarkers at the lower levels. MXene is a two-dimensional layered transition metal, attractive for biosensing due to its chemical, physical and electrical properties along with the biocompatibility. Materials and Methods: This work was focused on diagnosing osteosarcoma (OS), a common bone cancer, on MXene-modified multiple junction triangles by dielectrode sensing. Survivin protein gene is highly correlated with OS, identified on this sensing surface. Capture DNA was immobilized on MXene by using 3-glycidoxypropyltrimethox-ysilane as an amine linker and duplexed by the target DNA sequence. Results: The limitation and sensitivity of detection were found as 1 fM with the acceptable regression co-efficient value (y=1.0037⨰ + 0.525; R2=0.978) and the current enhancement was noted when increasing the target DNA concentrations. Moreover, the control sequences of single-and triple-mismatched and noncomplementary to the target DNA sequences failed to hybridize on the capture DNA, confirming the specificity. In addition, different batches were prepared with capture probe immobilized sensing surfaces and proved the efficient reproducibility. Conclusion: This microgap device with Mxene-modified multiple junction triangles dielec-trode surface is beneficial to quantify the survivin gene at its lower level and diagnosing OS complication levels. © 2020 Zhou et al. Dove Medical Press Ltd 2020 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85098218768&doi=10.2147%2fIJN.S284752&partnerID=40&md5=9c8e2699d1af1ab60f7f08f20edebada Zhou, D. and Gopinath, S.C.B. and Mohamed Saheed, M.S. and Siva Sangu, S. and Lakshmipriya, T. (2020) Mxene surface on multiple junction triangles for determining osteosarcoma cancer biomarker by dielectrode microgap sensor. International Journal of Nanomedicine, 15 . pp. 10171-10181. http://eprints.utp.edu.my/32408/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description Background: In recent years, nanomaterials have justified their dissemination for biosensor application towards the sensitive and selective detections of clinical biomarkers at the lower levels. MXene is a two-dimensional layered transition metal, attractive for biosensing due to its chemical, physical and electrical properties along with the biocompatibility. Materials and Methods: This work was focused on diagnosing osteosarcoma (OS), a common bone cancer, on MXene-modified multiple junction triangles by dielectrode sensing. Survivin protein gene is highly correlated with OS, identified on this sensing surface. Capture DNA was immobilized on MXene by using 3-glycidoxypropyltrimethox-ysilane as an amine linker and duplexed by the target DNA sequence. Results: The limitation and sensitivity of detection were found as 1 fM with the acceptable regression co-efficient value (y=1.0037⨰ + 0.525; R2=0.978) and the current enhancement was noted when increasing the target DNA concentrations. Moreover, the control sequences of single-and triple-mismatched and noncomplementary to the target DNA sequences failed to hybridize on the capture DNA, confirming the specificity. In addition, different batches were prepared with capture probe immobilized sensing surfaces and proved the efficient reproducibility. Conclusion: This microgap device with Mxene-modified multiple junction triangles dielec-trode surface is beneficial to quantify the survivin gene at its lower level and diagnosing OS complication levels. © 2020 Zhou et al.
format Article
author Zhou, D.
Gopinath, S.C.B.
Mohamed Saheed, M.S.
Siva Sangu, S.
Lakshmipriya, T.
spellingShingle Zhou, D.
Gopinath, S.C.B.
Mohamed Saheed, M.S.
Siva Sangu, S.
Lakshmipriya, T.
Mxene surface on multiple junction triangles for determining osteosarcoma cancer biomarker by dielectrode microgap sensor
author_sort Zhou, D.
title Mxene surface on multiple junction triangles for determining osteosarcoma cancer biomarker by dielectrode microgap sensor
title_short Mxene surface on multiple junction triangles for determining osteosarcoma cancer biomarker by dielectrode microgap sensor
title_full Mxene surface on multiple junction triangles for determining osteosarcoma cancer biomarker by dielectrode microgap sensor
title_fullStr Mxene surface on multiple junction triangles for determining osteosarcoma cancer biomarker by dielectrode microgap sensor
title_full_unstemmed Mxene surface on multiple junction triangles for determining osteosarcoma cancer biomarker by dielectrode microgap sensor
title_sort mxene surface on multiple junction triangles for determining osteosarcoma cancer biomarker by dielectrode microgap sensor
publisher Dove Medical Press Ltd
publishDate 2020
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85098218768&doi=10.2147%2fIJN.S284752&partnerID=40&md5=9c8e2699d1af1ab60f7f08f20edebada
http://eprints.utp.edu.my/32408/
_version_ 1741197729650966528
score 11.62408

Similar Items