Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review

5-fluorouracil (5-FU) refers to a fluorinated pyrimidine analogue that has been widely used as an anticancer agent for colon, head, and neck cancers. Detection of 5-FU and its metabolites; 5-fluorouridine and 5-fluoro-2-deoxyuridine in biological samples allows optimization of pharmacotherapy and en...

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Main Authors: Semail, N.-F., Abdul Keyon, A.S., Saad, B., Noordin, S.S., Nik Mohamed Kamal, N.N.S., Mohamad Zain, N.N., Azizi, J., Kamaruzaman, S., Yahaya, N.
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Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.29914 /
Published: Bellwether Publishing, Ltd. 2020
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087640894&doi=10.1080%2f10826076.2020.1781654&partnerID=40&md5=0411094d07b6b56c381025493618f166
http://eprints.utp.edu.my/29914/
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spelling utp-eprints.299142022-03-25T03:14:30Z Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review Semail, N.-F. Abdul Keyon, A.S. Saad, B. Noordin, S.S. Nik Mohamed Kamal, N.N.S. Mohamad Zain, N.N. Azizi, J. Kamaruzaman, S. Yahaya, N. 5-fluorouracil (5-FU) refers to a fluorinated pyrimidine analogue that has been widely used as an anticancer agent for colon, head, and neck cancers. Detection of 5-FU and its metabolites; 5-fluorouridine and 5-fluoro-2-deoxyuridine in biological samples allows optimization of pharmacotherapy and encourages fundamental investigations of this medication. The development of accurate and reliable sample preparation, as well as analytical methods, is critical to isolate targeted analytes from complex matrices, apart from increasing detection sensitivity of analytes. With that, this paper presents a review of prior studies pertaining to chromatographic and electrophoretic methods that focused on the analysis of 5-FU and its metabolites in biological matrices such as plasma and urine. This paper concentrates on HPLC, GC and CE systems, which are the most commonly used strategies for analytical separation of 5-FU and its metabolites from samples. Detection of these antineoplastic agents at trace level demands highly sensitive and selective analytical methodologies. Application of these analytical techniques to biological matrices is reviewed with a focus on method development strategies, including types of mobile phases and background electrolytes employed in LC and CE systems. © 2020 Taylor & Francis Group, LLC. Bellwether Publishing, Ltd. 2020 Article NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087640894&doi=10.1080%2f10826076.2020.1781654&partnerID=40&md5=0411094d07b6b56c381025493618f166 Semail, N.-F. and Abdul Keyon, A.S. and Saad, B. and Noordin, S.S. and Nik Mohamed Kamal, N.N.S. and Mohamad Zain, N.N. and Azizi, J. and Kamaruzaman, S. and Yahaya, N. (2020) Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review. Journal of Liquid Chromatography and Related Technologies, 43 (15-16). pp. 562-579. http://eprints.utp.edu.my/29914/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description 5-fluorouracil (5-FU) refers to a fluorinated pyrimidine analogue that has been widely used as an anticancer agent for colon, head, and neck cancers. Detection of 5-FU and its metabolites; 5-fluorouridine and 5-fluoro-2-deoxyuridine in biological samples allows optimization of pharmacotherapy and encourages fundamental investigations of this medication. The development of accurate and reliable sample preparation, as well as analytical methods, is critical to isolate targeted analytes from complex matrices, apart from increasing detection sensitivity of analytes. With that, this paper presents a review of prior studies pertaining to chromatographic and electrophoretic methods that focused on the analysis of 5-FU and its metabolites in biological matrices such as plasma and urine. This paper concentrates on HPLC, GC and CE systems, which are the most commonly used strategies for analytical separation of 5-FU and its metabolites from samples. Detection of these antineoplastic agents at trace level demands highly sensitive and selective analytical methodologies. Application of these analytical techniques to biological matrices is reviewed with a focus on method development strategies, including types of mobile phases and background electrolytes employed in LC and CE systems. © 2020 Taylor & Francis Group, LLC.
format Article
author Semail, N.-F.
Abdul Keyon, A.S.
Saad, B.
Noordin, S.S.
Nik Mohamed Kamal, N.N.S.
Mohamad Zain, N.N.
Azizi, J.
Kamaruzaman, S.
Yahaya, N.
spellingShingle Semail, N.-F.
Abdul Keyon, A.S.
Saad, B.
Noordin, S.S.
Nik Mohamed Kamal, N.N.S.
Mohamad Zain, N.N.
Azizi, J.
Kamaruzaman, S.
Yahaya, N.
Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review
author_sort Semail, N.-F.
title Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review
title_short Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review
title_full Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review
title_fullStr Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review
title_full_unstemmed Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review
title_sort analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: an updated review
publisher Bellwether Publishing, Ltd.
publishDate 2020
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087640894&doi=10.1080%2f10826076.2020.1781654&partnerID=40&md5=0411094d07b6b56c381025493618f166
http://eprints.utp.edu.my/29914/
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score 11.62408

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