Retention of Bioactive Compound in the Concentration of Centella Asiatica Extract through Progressive Freezing

Phenolics and flavonoids were the two major bioactive compounds in C.asiatica that contributes to its therapeutic values. However, concentration by evaporation resulted in denaturation of the compounds due to their heat sensitivity nature. Therefore, this paper aims to introduce progressive freezing...

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Main Authors: Amran, N.A., Mohd Rafee, N.H., Samsuri, S.
Format: Conference or Workshop Item
Institution: Universiti Teknologi Petronas
Record Id / ISBN-0: utp-eprints.24593 /
Published: Institute of Physics Publishing 2020
Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084289253&doi=10.1088%2f1757-899X%2f778%2f1%2f012175&partnerID=40&md5=a45813644596e6b4386ec496a4bfd47f
http://eprints.utp.edu.my/24593/
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spelling utp-eprints.245932021-08-27T05:26:12Z Retention of Bioactive Compound in the Concentration of Centella Asiatica Extract through Progressive Freezing Amran, N.A. Mohd Rafee, N.H. Samsuri, S. Phenolics and flavonoids were the two major bioactive compounds in C.asiatica that contributes to its therapeutic values. However, concentration by evaporation resulted in denaturation of the compounds due to their heat sensitivity nature. Therefore, this paper aims to introduce progressive freezing (PF) process as a new concentration method for the C.asiatica. PF separates two liquid compounds based on their freezing point difference. One with a higher freezing point would solidify first while another one would remain as a liquid. Low temperature involved in this process could minimize the heat abuse to the volatile compounds of C.asiatica. In this study, maceration was done to get the C.asiatica extract by preparing 1:80 ratio of solute to solvent and stirred at 1200 rpm for 24 hours. On the other hand, the PF was conducted in a cylindrical stirred crystallizer. For PF process, two operating parameters which are coolant temperature ranging from -6°C to -12°C, and stirring rate ranging from 100 rpm to 250 rpm have been tested for their effects towards the process performance. Two determinant parameters which were effective partition constant, K and retention efficiency (Reff) of bioactive compounds (flavonoid and phenolic) in the concentrated solution had been used to portray the process efficiency. On completing the experimental work, it was found that lower coolant temperature, and a moderate stirring rate resulted in the higher concentration efficiency of the bioactive compounds. The highest concentration efficiency of 69 and the smallest effective partition constant (K) of 0.51 were achieved at -10°C, 150 rpm and 30 minutes. © 2020 IOP Publishing Ltd. Institute of Physics Publishing 2020 Conference or Workshop Item NonPeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084289253&doi=10.1088%2f1757-899X%2f778%2f1%2f012175&partnerID=40&md5=a45813644596e6b4386ec496a4bfd47f Amran, N.A. and Mohd Rafee, N.H. and Samsuri, S. (2020) Retention of Bioactive Compound in the Concentration of Centella Asiatica Extract through Progressive Freezing. In: UNSPECIFIED. http://eprints.utp.edu.my/24593/
institution Universiti Teknologi Petronas
collection UTP Institutional Repository
description Phenolics and flavonoids were the two major bioactive compounds in C.asiatica that contributes to its therapeutic values. However, concentration by evaporation resulted in denaturation of the compounds due to their heat sensitivity nature. Therefore, this paper aims to introduce progressive freezing (PF) process as a new concentration method for the C.asiatica. PF separates two liquid compounds based on their freezing point difference. One with a higher freezing point would solidify first while another one would remain as a liquid. Low temperature involved in this process could minimize the heat abuse to the volatile compounds of C.asiatica. In this study, maceration was done to get the C.asiatica extract by preparing 1:80 ratio of solute to solvent and stirred at 1200 rpm for 24 hours. On the other hand, the PF was conducted in a cylindrical stirred crystallizer. For PF process, two operating parameters which are coolant temperature ranging from -6°C to -12°C, and stirring rate ranging from 100 rpm to 250 rpm have been tested for their effects towards the process performance. Two determinant parameters which were effective partition constant, K and retention efficiency (Reff) of bioactive compounds (flavonoid and phenolic) in the concentrated solution had been used to portray the process efficiency. On completing the experimental work, it was found that lower coolant temperature, and a moderate stirring rate resulted in the higher concentration efficiency of the bioactive compounds. The highest concentration efficiency of 69 and the smallest effective partition constant (K) of 0.51 were achieved at -10°C, 150 rpm and 30 minutes. © 2020 IOP Publishing Ltd.
format Conference or Workshop Item
author Amran, N.A.
Mohd Rafee, N.H.
Samsuri, S.
spellingShingle Amran, N.A.
Mohd Rafee, N.H.
Samsuri, S.
Retention of Bioactive Compound in the Concentration of Centella Asiatica Extract through Progressive Freezing
author_sort Amran, N.A.
title Retention of Bioactive Compound in the Concentration of Centella Asiatica Extract through Progressive Freezing
title_short Retention of Bioactive Compound in the Concentration of Centella Asiatica Extract through Progressive Freezing
title_full Retention of Bioactive Compound in the Concentration of Centella Asiatica Extract through Progressive Freezing
title_fullStr Retention of Bioactive Compound in the Concentration of Centella Asiatica Extract through Progressive Freezing
title_full_unstemmed Retention of Bioactive Compound in the Concentration of Centella Asiatica Extract through Progressive Freezing
title_sort retention of bioactive compound in the concentration of centella asiatica extract through progressive freezing
publisher Institute of Physics Publishing
publishDate 2020
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084289253&doi=10.1088%2f1757-899X%2f778%2f1%2f012175&partnerID=40&md5=a45813644596e6b4386ec496a4bfd47f
http://eprints.utp.edu.my/24593/
_version_ 1741196837953470464
score 11.62408

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